Detailed information of TA system TA219765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 604922..605451
• Replicon: chromosome
• Accession: NZ_CP084878
• Organism: Staphylococcus aureus strain GS426

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LD199_RS02855
• Protein ID: WP_000621175.1
• Coordinates: 605089..605451 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LD199_RS02850
• Protein ID: WP_000948331.1
• Coordinates: 604922..605092 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD199_RS02820 (LD199_02820)	599959..600519	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
LD199_RS02825 (LD199_02825)	600727..601206	+	480	WP_001287087.1	hypothetical protein	-
LD199_RS02830 (LD199_02830)	601199..602782	+	1584	WP_001294626.1	PH domain-containing protein	-
LD199_RS02835 (LD199_02835)	602769..603260	+	492	WP_001205912.1	PH domain-containing protein	-
LD199_RS02840 (LD199_02840)	603264..603623	+	360	WP_000581197.1	holo-ACP synthase	-
LD199_RS02845 (LD199_02845)	603689..604837	+	1149	WP_001281154.1	alanine racemase	-
LD199_RS02850 (LD199_02850)	604922..605092	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LD199_RS02855 (LD199_02855)	605089..605451	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LD199_RS02860 (LD199_02860)	605801..606802	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LD199_RS02865 (LD199_02865)	606921..607247	+	327	WP_001052491.1	anti-sigma factor antagonist	-
LD199_RS02870 (LD199_02870)	607249..607728	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
LD199_RS02875 (LD199_02875)	607703..608473	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T219765 WP_000621175.1 NZ_CP084878:605089-605451 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T219765 NZ_CP116692:c539820-539521 [Agrobacterium tumefaciens]
ATGGCCGTGACGGAATTTAGCAAATTCGATGTGTCAGACTATCTGGATAGCGATGAAACCATGATCGAATATCTGGCTGT
CGCGATGGAAGATGAAAACCCCGATGTCTTCATCGCGGCCCTTGGCGCAGTCGCCCGCGCGCGTGGAATGTCGCACGTCG
CGGATGTTTCGGGCTTGGGGCGCGAAAGCCTCTATAAAGCGCTCAGGCCGGGTTCAAAGCTGCGCTACGAAACCGTTCAG
AGGGTGTTGCAGGCGCTCGGCTTCCGGCTGGCCGTCGAAAAATCGCTGAACGCCGCGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT219765 WP_000948331.1 NZ_CP084878:604922-605092 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT219765 NZ_CP116692:c540067-539801 [Agrobacterium tumefaciens]
TTGAAAGACCGCACAGGAAAAGTGCGGATTCTACATCGTCTCACGGCGGCAGAGCATGGGAACTTCGGTGATTGTGAGCC
TGTGGGTGGCGGCGTTTCGGAAATGCGTATCCACCACGGTCCGGGTTATCGGGTCTATTTCACCCGCATTGGCAAGACAA
TCTATTTCCTTCTTGTCGGCGGCGACAAGTCTTCGCAGAAACGGGACATCAAGCGCGCAATCGAGATGGCGCAAACCATA
GGAAGGGATGGCCGTGACGGAATTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1