Detailed information of TA system TA219759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 48889..49625
• Replicon: plasmid pKqq_01A030T_1
• Accession: NZ_CP084877
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain 01A030T

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: LGM20_RS25810
• Protein ID: WP_004187044.1
• Coordinates: 49143..49625 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LGM20_RS25805
• Protein ID: WP_003026799.1
• Coordinates: 48889..49155 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM20_RS25785 (LGM20_25785)	46222..47175	-	954	WP_004206586.1	ferric citrate uptake sigma factor regulator FecR	-
LGM20_RS25790 (LGM20_25790)	47172..47693	-	522	WP_004206587.1	RNA polymerase sigma factor FecI	-
LGM20_RS25795 (LGM20_25795)	47830..47899	+	70	Protein_50	hypothetical protein	-
LGM20_RS25800 (LGM20_25800)	48034..48738	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LGM20_RS25805 (LGM20_25805)	48889..49155	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LGM20_RS25810 (LGM20_25810)	49143..49625	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
LGM20_RS25815 (LGM20_25815)	49832..51178	+	1347	WP_077253535.1	ISNCY family transposase	-
LGM20_RS25820 (LGM20_25820)	51227..51622	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
LGM20_RS25825 (LGM20_25825)	51811..53208	+	1398	WP_009653207.1	ISNCY family transposase	-
LGM20_RS25830 (LGM20_25830)	53229..53420	+	192	Protein_57	hypothetical protein	-
LGM20_RS25835 (LGM20_25835)	53448..53726	-	279	WP_004197671.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / qacE / sul1	-	1..163751	163751
	-	inside	IScluster/Tn	-	-	48034..53208	5174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T219759 WP_004187044.1 NZ_CP084877:49143-49625 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T219759 NZ_CP116688:c574846-574511 [Agrobacterium tumefaciens]
GTGACGGATAAAAAAGGCCACGGAAAAGATGCGGCCGCCAAGCGGACCGTGCTGCCGCGTAGTTCGGATTTCACCAAGCA
ATTTATTAAAGACTGGCAACGGCTGAACAATTCCGGGCGTTACGACATGGTGAAACTGAAGGAGATCATGCTCCTTCTCA
TCGCCAATGAAGCGCCTTTACCGGCGCAGTTTCGTGACCATGAACTGACAGGAGAATGGCACGATCATCGCGAATGCCAT
GTCGGCGGGAACTTTCTGCTGATCTACAAACTTGATGAGAAGCAGAACTTTCTCATCTTTACCCGCGCAGGGACGCACGC
CGAGCTCTTCCGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT219759 WP_003026799.1 NZ_CP084877:48889-49155 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT219759 NZ_CP116688:c575118-574843 [Agrobacterium tumefaciens]
ATGACCGCAAATGCTTATGTGAGAGCGCGCATCGATCCGGCACTCAAGGATGACGCCACTGCCGTACTCGATAGTCTGGG
ATTGACTGTTTCCGATGTCATGCGAATGATGCTGACGCGCATTGCCCGAGAAAAAGCACTGCCGATAGAACTGACACAGC
CAAACGCTGAAACGCTTGCCGCGATTGAAGAAGCGCGCGCCATCGCGGCCGCCAGGCACAATCGCTTCGTCACGGCGGAA
GCCTTGTTTGAGGCCCTTGATGGTAACAAACGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66