Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3966136..3966755 Replicon chromosome
Accession NZ_CP084874
Organism Klebsiella africana strain 200023

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGL98_RS19130 Protein ID WP_002892050.1
Coordinates 3966537..3966755 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A223UF87
Locus tag LGL98_RS19125 Protein ID WP_025710692.1
Coordinates 3966136..3966510 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL98_RS19115 (LGL98_19115) 3961289..3962482 + 1194 WP_136033418.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGL98_RS19120 (LGL98_19120) 3962505..3965651 + 3147 WP_136033420.1 multidrug efflux RND transporter permease subunit AcrB -
LGL98_RS19125 (LGL98_19125) 3966136..3966510 + 375 WP_025710692.1 Hha toxicity modulator TomB Antitoxin
LGL98_RS19130 (LGL98_19130) 3966537..3966755 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LGL98_RS19135 (LGL98_19135) 3966913..3967479 + 567 WP_136033421.1 maltose O-acetyltransferase -
LGL98_RS19140 (LGL98_19140) 3967451..3967579 - 129 Protein_3749 hypothetical protein -
LGL98_RS19145 (LGL98_19145) 3967616..3968086 + 471 WP_025710690.1 YlaC family protein -
LGL98_RS19150 (LGL98_19150) 3968061..3969512 - 1452 WP_136033423.1 PLP-dependent aminotransferase family protein -
LGL98_RS19155 (LGL98_19155) 3969613..3970311 + 699 WP_136033425.1 GNAT family protein -
LGL98_RS19160 (LGL98_19160) 3970308..3970448 - 141 WP_136033621.1 type B 50S ribosomal protein L36 -
LGL98_RS19165 (LGL98_19165) 3970448..3970711 - 264 WP_080924893.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219742 WP_002892050.1 NZ_CP084874:3966537-3966755 [Klebsiella africana]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219742 NZ_CP116680:471427-471816 [Acinetobacter baumannii Canada BC-5]
TTGCTCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATAAGCTTTATTTTCTTTT
TAAAGCGTCCGCAACTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14398.04 Da        Isoelectric Point: 4.7295

>AT219742 WP_025710692.1 NZ_CP084874:3966136-3966510 [Klebsiella africana]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIDQVDEYL
DDTFTLFSNYGINSSDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219742 NZ_CP116680:471163-471420 [Acinetobacter baumannii Canada BC-5]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A223UF87

References