Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1780110..1780700 Replicon chromosome
Accession NZ_CP084874
Organism Klebsiella africana strain 200023

Toxin (Protein)


Gene name pemK Uniprot ID A0A8B6IZR4
Locus tag LGL98_RS08555 Protein ID WP_040245064.1
Coordinates 1780368..1780700 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag LGL98_RS08550 Protein ID WP_000288812.1
Coordinates 1780110..1780367 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL98_RS08525 (LGL98_08525) 1775931..1776017 + 87 Protein_1666 type II toxin-antitoxin system PemK/MazF family toxin -
LGL98_RS08530 (LGL98_08530) 1776368..1778116 + 1749 WP_226651828.1 hypothetical protein -
LGL98_RS08535 (LGL98_08535) 1778195..1778752 + 558 Protein_1668 hypothetical protein -
LGL98_RS08540 (LGL98_08540) 1779111..1779572 + 462 WP_136032146.1 hypothetical protein -
LGL98_RS08545 (LGL98_08545) 1779569..1779775 + 207 WP_136032094.1 helix-turn-helix transcriptional regulator -
LGL98_RS08550 (LGL98_08550) 1780110..1780367 + 258 WP_000288812.1 antitoxin Antitoxin
LGL98_RS08555 (LGL98_08555) 1780368..1780700 + 333 WP_040245064.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LGL98_RS08570 (LGL98_08570) 1781022..1782458 + 1437 WP_168435312.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LGL98_RS08580 (LGL98_08580) 1782784..1782933 - 150 Protein_1674 antitoxin -
LGL98_RS08585 (LGL98_08585) 1783137..1783439 - 303 WP_061153979.1 XRE family transcriptional regulator -
LGL98_RS08590 (LGL98_08590) 1783444..1783797 - 354 WP_008804168.1 type II toxin-antitoxin system RelE/ParE family toxin -
LGL98_RS08595 (LGL98_08595) 1784210..1784668 + 459 WP_136032098.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11885.75 Da        Isoelectric Point: 10.1839

>T219730 WP_040245064.1 NZ_CP084874:1780368-1780700 [Klebsiella africana]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAMLS

Download         Length: 333 bp

>T219730 NZ_CP116672:c4770050-4769916 [Xanthomonas hortorum pv. pelargonii]
ATGAAGCGTGCAATTATGCTGCTGGTGCTGTCGGTGTTTTCGGTCGGTATGTTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAAGTGGAAGACGCTGCGCGCAAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT219730 WP_000288812.1 NZ_CP084874:1780110-1780367 [Klebsiella africana]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT219730 NZ_CP116672:c4770281-4770129 [Xanthomonas hortorum pv. pelargonii]
ATGAAGCGACTGCTGACACTGATGGTGCTAGGCCTGTTTTCGGCTGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
CGGCAAGGACATGCAGGGCGCAGGTGAGAAGGTCGAAAAGAAGGCCGATAATTGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6IZR4

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References