Detailed information of TA system TA021973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 523296..523737
• Replicon: chromosome
• Accession: NC_009078
• Organism: Burkholderia pseudomallei 1106a

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BURPS1106A_RS30180
• Protein ID: WP_009939402.1
• Coordinates: 523296..523469 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BURPS1106A_RS19820
• Protein ID: WP_004525420.1
• Coordinates: 523453..523737 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BURPS1106A_RS39020	518895..519323	-	429	Protein_421	DDE-type integrase/transposase/recombinase	-
BURPS1106A_RS39025	519321..520094	-	774	Protein_422	prohibitin family protein	-
BURPS1106A_RS19785	520091..520240	-	150	WP_004537646.1	bacteriophage protein Gp44	-
BURPS1106A_RS19790	520490..521080	+	591	WP_009946442.1	hypothetical protein	-
BURPS1106A_RS39030	521255..521473	-	219	WP_004549687.1	hypothetical protein	-
BURPS1106A_RS19800	521521..521868	-	348	WP_009946438.1	hypothetical protein	-
BURPS1106A_RS39035	521897..522073	-	177	WP_004552958.1	hypothetical protein	-
BURPS1106A_RS19815	522353..522628	-	276	WP_004537565.1	bacteriophage protein Gp49	-
BURPS1106A_RS30180	523296..523469	+	174	WP_009939402.1	BrnT family toxin	Toxin
BURPS1106A_RS19820	523453..523737	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BURPS1106A_RS19830	524384..524767	+	384	Protein_431	DDE-type integrase/transposase/recombinase	-
BURPS1106A_RS34525	524730..525158	+	429	Protein_432	IS110 family transposase	-
BURPS1106A_RS19835	525385..525501	-	117	Protein_433	IS3 family transposase	-
BURPS1106A_RS19840	525767..527227	+	1461	WP_004536920.1	metallophosphoesterase	-
BURPS1106A_RS19845	527386..528648	+	1263	WP_004537187.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	516584..527227	10643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T21973 WP_009939402.1 NC_009078:523296-523469 [Burkholderia pseudomallei 1106a]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T21973 NC_009078:523296-523469 [Burkholderia pseudomallei 1106a]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT21973 WP_004525420.1 NC_009078:523453-523737 [Burkholderia pseudomallei 1106a]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT21973 NC_009078:523453-523737 [Burkholderia pseudomallei 1106a]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8