Detailed information of TA system TA219728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 743886..744661
• Replicon: chromosome
• Accession: NZ_CP084874
• Organism: Klebsiella africana strain 200023

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LGL98_RS03660
• Protein ID: WP_136031433.1
• Coordinates: 744176..744661 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7U2ZSW0
• Locus tag: LGL98_RS03655
• Protein ID: WP_025710477.1
• Coordinates: 743886..744179 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL98_RS03635 (LGL98_03635)	739091..739693	-	603	WP_136031428.1	short chain dehydrogenase	-
LGL98_RS03640 (LGL98_03640)	739791..740702	+	912	WP_014906929.1	LysR family transcriptional regulator	-
LGL98_RS03645 (LGL98_03645)	740703..741851	-	1149	WP_136031430.1	PLP-dependent aspartate aminotransferase family protein	-
LGL98_RS03650 (LGL98_03650)	741862..743238	-	1377	WP_168435287.1	cystathionine beta-synthase	-
LGL98_RS03655 (LGL98_03655)	743886..744179	+	294	WP_025710477.1	DUF1778 domain-containing protein	Antitoxin
LGL98_RS03660 (LGL98_03660)	744176..744661	+	486	WP_136031433.1	GNAT family N-acetyltransferase	Toxin
LGL98_RS03665 (LGL98_03665)	745367..745960	+	594	WP_046881922.1	hypothetical protein	-
LGL98_RS03670 (LGL98_03670)	746057..746273	+	217	Protein_722	transposase	-
LGL98_RS03680 (LGL98_03680)	746951..747664	-	714	WP_136031435.1	DUF554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	746057..746185	128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17480.41 Da
• Isoelectric Point: 8.2962

>T219728 WP_136031433.1 NZ_CP084874:744176-744661 [Klebsiella africana]
MISAPEPLHAGHILTPFCSGVDSMDNWLKQRAMKNQLSGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVEKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLGDLVH
A

Nucleotide

• Length: 486 bp

>T219728 NZ_CP116672:3881634-3881891 [Xanthomonas hortorum pv. pelargonii]
GTGATTCTGCAGTTTGCTGACAATGCCTGGGAGGACTACCTCTACTGGCAACAGACCGACAAGAAGATGCTCAAACGCAT
CAACGAGTTGATCAAGGCCATTCAGCGCGATCCTTTCCAGGGCATCGGCAAACCCGAACCGTTGCGCCATGCGCTGGCGG
GCTACTGGTCACGCCGCATCAACGACGAACACCGGATTGTCTACAAGGCCGAGAACGGTATCCTGCTGATTGCTCAGGCG
CGCTACCACTACGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10990.61 Da
• Isoelectric Point: 8.6141

>AT219728 WP_025710477.1 NZ_CP084874:743886-744179 [Klebsiella africana]
MPAANSMTMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT219728 NZ_CP116672:3881386-3881637 [Xanthomonas hortorum pv. pelargonii]
ATGGACACCATCACTTACAGCGCCGCGCGTGCCTCTCTGGCCGACACCATGGACCGTGTCGTCAATGACCATGAGCCGGT
GATCATCACGCGCAGCGGTGAGCGCGCCGTAGTCATGTTGTCGCTGGAAGACTACAAGGCCATGGAAGAGACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAACGGCTGCTGGAATCCATTGCCCAGCTTGAGGCCGGTCGCGGCAAGGAACGGGAA
CTGGCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U2ZSW0