Detailed information of TA system TA219726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 291400..292031
• Replicon: chromosome
• Accession: NZ_CP084874
• Organism: Klebsiella africana strain 200023

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LGL98_RS01315
• Protein ID: WP_136033797.1
• Coordinates: 291400..291675 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: R4YIG2
• Locus tag: LGL98_RS01320
• Protein ID: WP_004181489.1
• Coordinates: 291672..292031 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL98_RS01295 (LGL98_01295)	286637..286972	+	336	WP_002921203.1	universal stress protein UspB	-
LGL98_RS01300 (LGL98_01300)	287032..288528	-	1497	WP_025710729.1	inorganic phosphate transporter PitA	-
LGL98_RS01305 (LGL98_01305)	288758..289951	+	1194	WP_136033801.1	NAD(P)/FAD-dependent oxidoreductase	-
LGL98_RS01310 (LGL98_01310)	289991..291004	-	1014	WP_136033799.1	magnesium transporter	-
LGL98_RS01315 (LGL98_01315)	291400..291675	+	276	WP_136033797.1	type II toxin-antitoxin system HicA family toxin	Toxin
LGL98_RS01320 (LGL98_01320)	291672..292031	+	360	WP_004181489.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LGL98_RS01325 (LGL98_01325)	292059..292460	-	402	WP_004181488.1	nickel-responsive transcriptional regulator NikR	-
LGL98_RS01330 (LGL98_01330)	292448..293239	-	792	WP_136033795.1	nickel import ATP-binding protein NikE	-
LGL98_RS01335 (LGL98_01335)	293236..294000	-	765	WP_136033793.1	nickel import ATP-binding protein NikD	-
LGL98_RS01340 (LGL98_01340)	294000..294833	-	834	WP_136033791.1	nickel ABC transporter permease subunit NikC	-
LGL98_RS01345 (LGL98_01345)	294830..295774	-	945	WP_136033789.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10270.90 Da
• Isoelectric Point: 10.3164

>T219726 WP_136033797.1 NZ_CP084874:291400-291675 [Klebsiella africana]
MEQQVLSLRNKQRQTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Nucleotide

• Length: 276 bp

>T219726 NZ_CP116672:2552124-2552330 [Xanthomonas hortorum pv. pelargonii]
ATGCAAGCTGTAGCCGAACGGAAGCTGCAATTGCTGGGTTCGGCGCAGACGCTGGAGTTCTTGCGCAGCCCGCCGGGCAA
CCGGCTTGAGCTGTTGGCAGGTACGCGCGCAGGTCAGCACAGCAGCCGCATCAATGACCAATGGCGGGTGTGCGTTGTCT
GGACTGACGCCGGCCCAGAACACGTTGAGATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13313.02 Da
• Isoelectric Point: 4.4605

>AT219726 WP_004181489.1 NZ_CP084874:291672-292031 [Klebsiella africana]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Nucleotide

• Length: 360 bp

>AT219726 NZ_CP116672:2552340-2552639 [Xanthomonas hortorum pv. pelargonii]
ATGCGGCCCACGAACAAACTGCGCGCCATCCATCCCGGCGAGATTCTGCGGAAAGACTTCATGCTGCCATTGGGGTTGAC
CATCAACGGCCTGGCGCGGGCGCTGGATGTACCGGCAACGCGCATCCACAGCATCGTGCACGGCGAGCGTGCCATCAGCG
CCGACACCGCGGCACGGCTTGCACGCCACTTCGGTGGCGATGCGGCGTCCTGGCTGGCGCTACGGGCCACCCACGATCTG
AAGACCTTGCCCAACCGCAGCGAGATCGAGCGCAATGTGCGGCCCCGTGTGGTGGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1F2M5Q1