Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5079567..5080192 Replicon chromosome
Accession NZ_CP084873
Organism Klebsiella pneumoniae strain 03-9138-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGL99_RS24685 Protein ID WP_002882817.1
Coordinates 5079567..5079950 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGL99_RS24690 Protein ID WP_004150355.1
Coordinates 5079950..5080192 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL99_RS24670 (5076933) 5076933..5077835 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGL99_RS24675 (5077832) 5077832..5078467 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGL99_RS24680 (5078464) 5078464..5079393 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGL99_RS24685 (5079567) 5079567..5079950 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGL99_RS24690 (5079950) 5079950..5080192 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGL99_RS24695 (5080397) 5080397..5081314 + 918 WP_029884108.1 alpha/beta hydrolase -
LGL99_RS24700 (5081328) 5081328..5082269 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGL99_RS24705 (5082314) 5082314..5082751 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGL99_RS24710 (5082748) 5082748..5083608 - 861 WP_002882807.1 virulence factor BrkB family protein -
LGL99_RS24715 (5083602) 5083602..5084201 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219725 WP_002882817.1 NZ_CP084873:c5079950-5079567 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219725 NZ_CP116672:c1461147-1460656 [Xanthomonas hortorum pv. pelargonii]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCGACCGGTGCGTCACGGACCTTTGTTGCCTGCGCGGATGACCGCGTGG
TGGCCTATTACGCCTTGGCCTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGCAGGCTTGCTGTCGACCAATCCCTGCACGGCAGAGGGTTTGGTCGCGCCCTGGTACGGGA
TGCAGGCAAGCGCGTCCTGCATGCAGCCGACACCATTGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGACGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219725 WP_004150355.1 NZ_CP084873:c5080192-5079950 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219725 NZ_CP116672:c1461434-1461144 [Xanthomonas hortorum pv. pelargonii]
ATGTCCACACCCAACACTACGCCCGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCCGAGGAACGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCGTGCTGGACGCGGCGCGCTCGGCTGCCGAGGAAGCCTTGC
TGGACCAGACCCTTATCGCGGCCAGCCCGGACGCCTACGCCGCCTTTCTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References