Detailed information of TA system TA219724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4755228..4756909
• Replicon: chromosome
• Accession: NZ_CP084873
• Organism: Klebsiella pneumoniae strain 03-9138-2

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: LGL99_RS23175
• Protein ID: WP_004146663.1
• Coordinates: 4755228..4756145 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: LGL99_RS23180
• Protein ID: WP_004177809.1
• Coordinates: 4756151..4756909 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL99_RS23155 (4750534)	4750534..4752054	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
LGL99_RS23160 (4752051)	4752051..4753049	+	999	WP_004872882.1	ribose ABC transporter permease	-
LGL99_RS23165 (4753080)	4753080..4754021	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LGL99_RS23170 (4754096)	4754096..4755103	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
LGL99_RS23175 (4755228)	4755228..4756145	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LGL99_RS23180 (4756151)	4756151..4756909	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LGL99_RS23185 (4756995)	4756995..4757723	-	729	WP_004177810.1	response regulator transcription factor	-
LGL99_RS23190 (4757996)	4757996..4760143	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
LGL99_RS23195 (4760605)	4760605..4761291	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T219724 WP_004146663.1 NZ_CP084873:c4756145-4755228 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T219724 NZ_CP116621:3904315-3904569 [Proteus terrae]
ATGAAATTAATTTTTTCTGAGCAATCATGGAGTGATTATCTTTATTGGCAACAAATAGACAAGAAAATAATTAAACGCAT
TAACGAACTCATAAAAGATATTAAAAGAACACCGTTTAGCGGAATAGGTAAACCAGAGCCCTTGAAACATAATTTAGCAG
GTTTTTGGTCAAGACGGATCACTGATGAACATCGCCTTATCTACCGTATTACTGATTCCGCTATAGAAATTGCATCATGT
CGATACCACTATTAG

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT219724 WP_004177809.1 NZ_CP084873:c4756909-4756151 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT219724 NZ_CP116621:3904067-3904318 [Proteus terrae]
ATGAAAACGATAAATTATACAGAAGCAAGACAAAACTTAACTTCAATTATGAATGAAACAATTGATGATAGAGTACCCAT
CATGATCACAAGGCAAAAGGGTATTCCATGTGTTCTTTTATCTCTTGATGAATACCAAGCACTAGAAGAAACTGCCTATC
TGTTACGATCTCCAGCGAATGCGCGCCATTTGCTCGATTCTATTGATGAACTGAACCAAGGTAAAGGTATAGAAAAGGAT
ATAACGGAATGA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05