Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4464769..4465579 Replicon chromosome
Accession NZ_CP084873
Organism Klebsiella pneumoniae strain 03-9138-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGL99_RS21810 Protein ID WP_004178461.1
Coordinates 4464769..4465302 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag LGL99_RS21815 Protein ID WP_004222378.1
Coordinates 4465313..4465579 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL99_RS21805 (4463600) 4463600..4464721 + 1122 WP_002887282.1 cupin domain-containing protein -
LGL99_RS21810 (4464769) 4464769..4465302 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGL99_RS21815 (4465313) 4465313..4465579 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGL99_RS21820 (4465682) 4465682..4467115 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
LGL99_RS21825 (4467105) 4467105..4467788 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LGL99_RS21830 (4467960) 4467960..4469345 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
LGL99_RS21835 (4469363) 4469363..4469707 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219723 WP_004178461.1 NZ_CP084873:c4465302-4464769 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219723 NZ_CP116621:3904067-3904318 [Proteus terrae]
ATGAAAACGATAAATTATACAGAAGCAAGACAAAACTTAACTTCAATTATGAATGAAACAATTGATGATAGAGTACCCAT
CATGATCACAAGGCAAAAGGGTATTCCATGTGTTCTTTTATCTCTTGATGAATACCAAGCACTAGAAGAAACTGCCTATC
TGTTACGATCTCCAGCGAATGCGCGCCATTTGCTCGATTCTATTGATGAACTGAACCAAGGTAAAGGTATAGAAAAGGAT
ATAACGGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT219723 WP_004222378.1 NZ_CP084873:c4465579-4465313 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219723 NZ_CP116621:3904315-3904569 [Proteus terrae]
ATGAAATTAATTTTTTCTGAGCAATCATGGAGTGATTATCTTTATTGGCAACAAATAGACAAGAAAATAATTAAACGCAT
TAACGAACTCATAAAAGATATTAAAAGAACACCGTTTAGCGGAATAGGTAAACCAGAGCCCTTGAAACATAATTTAGCAG
GTTTTTGGTCAAGACGGATCACTGATGAACATCGCCTTATCTACCGTATTACTGATTCCGCTATAGAAATTGCATCATGT
CGATACCACTATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References