Detailed information of TA system TA219710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 333683..334269
• Replicon: chromosome
• Accession: NZ_CP084873
• Organism: Klebsiella pneumoniae strain 03-9138-2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A7Z9B6M3
• Locus tag: LGL99_RS01545
• Protein ID: WP_029884514.1
• Coordinates: 333901..334269 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LGL99_RS01540
• Protein ID: WP_004174006.1
• Coordinates: 333683..333904 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL99_RS01520 (329840)	329840..330766	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LGL99_RS01525 (330763)	330763..332040	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LGL99_RS01530 (332037)	332037..332804	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LGL99_RS01535 (332806)	332806..333519	+	714	WP_029884513.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LGL99_RS01540 (333683)	333683..333904	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LGL99_RS01545 (333901)	333901..334269	+	369	WP_029884514.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LGL99_RS01550 (334542)	334542..335858	+	1317	WP_029884515.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LGL99_RS01555 (335965)	335965..336852	+	888	WP_004901104.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LGL99_RS01560 (336849)	336849..337694	+	846	WP_004901101.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LGL99_RS01565 (337696)	337696..338766	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	292349..339503	47154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-116)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13583.01 Da
• Isoelectric Point: 8.6410

>T219710 WP_029884514.1 NZ_CP084873:333901-334269 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRMALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T219710 NZ_CP116604:c2320343-2320014 [Streptococcus sp. HN38]
ATGGAATTTGAGTCATATTCAGTGATGCTTGCTCCAGCAGTGGAGAAGGAATTGGCAGTTATTTACGCCTATTTCTCGGA
ACAGTTCTCAGAAGAAATTGCCAAAAGACGAATTGGGATGATTGTCGAAGCATTGGAGAGTTTGCAAATTTTTCCTGAAC
GTGGCTTTAACGCCGATAACCGTTTTGGTAAACAGATAGACCCGCCTCATCTGACCCGAGGCTATGTCATTGGAAAAGAC
TACATTGCCCTCTATCGCGTGGTAGAAAATGAAGTGCGAGTTGGTCACCTTTTTGCGACCAAGAGTGATTATGTCAAGTT
GTTGAAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT219710 WP_004174006.1 NZ_CP084873:333683-333904 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT219710 NZ_CP116604:c2320620-2320333 [Streptococcus sp. HN38]
TTGATAACTATAAAAAAAGATGCCCAAGTAAATTTTAAAACTGACTCCAGACTTTTGGAAAAAGCCAAGGAAATATTTGC
TATGAACCAGTTGGATTTGACGGCTGGTTTCAATCTCTTTTTGCAAAACATTGCTGTAAAAAATGAGTTGCCAATTCTGA
CTGAAGAGGAATTGGAAAGAGAAGAGTTATTTTTACAGCTTCAGAAGGAAATACAGGAAAACCAACAGGCGATTGAGGCA
GGTCAAGGGATCAGCCTAGAAAGTGTCAAAGAGAAGTATGGAATTTGA

Structures

Toxin

• AlphaFold DB: A0A7Z9B6M3

Antitoxin

• AlphaFold DB: A0A5E5YJY7