Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5273774..5274399 Replicon chromosome
Accession NZ_CP084871
Organism Klebsiella pneumoniae strain 04A025

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGM00_RS25585 Protein ID WP_002882817.1
Coordinates 5273774..5274157 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM00_RS25590 Protein ID WP_004150355.1
Coordinates 5274157..5274399 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM00_RS25570 (5271140) 5271140..5272042 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGM00_RS25575 (5272039) 5272039..5272674 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGM00_RS25580 (5272671) 5272671..5273600 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGM00_RS25585 (5273774) 5273774..5274157 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM00_RS25590 (5274157) 5274157..5274399 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM00_RS25595 (5274604) 5274604..5275521 + 918 WP_004181612.1 alpha/beta hydrolase -
LGM00_RS25600 (5275535) 5275535..5276476 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM00_RS25605 (5276521) 5276521..5276958 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGM00_RS25610 (5276955) 5276955..5277815 - 861 WP_004146232.1 virulence factor BrkB family protein -
LGM00_RS25615 (5277809) 5277809..5278408 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219708 WP_002882817.1 NZ_CP084871:c5274157-5273774 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219708 NZ_CP116604:c2194752-2194492 [Streptococcus sp. HN38]
ATGTTTCATGTAGAGTATTCTAAGAAGGCACAGAGACAAATCAGAAAATTGGATAAGCCAATCCAAAGATTATTATTTGC
ATGGGTGGATAAACACTTAGATGGTACAGTTAATCCGAGGTTGCATGGCAAGGGATTGACAGGGGACCATGCCAATGAGT
GGCGTTATCGTATCGGTGATTATCGGCTGATTTGCGATATACAGGATGATAGGATGGTTATTCTGGCACTTGAATTTGGT
CATCGTAGAGATATTTACTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219708 WP_004150355.1 NZ_CP084871:c5274399-5274157 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219708 NZ_CP116604:c2194975-2194754 [Streptococcus sp. HN38]
ATGAGTACAGTAACTATCCGATTAAACAAAGAGGAAGAAGTATTTTTCAAGAGCTATGCTCAATTGACTGGTCAAAGTCT
TTCAAGTTTATTTAAGAAGGCACTTGAGCGTGATATTGAGGATGAGTATGATTTGAACATTTACCATCAAGCCTACGAGG
AGTACCAGAAAGATCCTGAAACCATCAGTCATGCGGATTTCAAGAAGGAACTTGGTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References