Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4694410..4695220 Replicon chromosome
Accession NZ_CP084871
Organism Klebsiella pneumoniae strain 04A025

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGM00_RS22905 Protein ID WP_004178461.1
Coordinates 4694410..4694943 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LGM00_RS22910 Protein ID WP_002887278.1
Coordinates 4694954..4695220 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM00_RS22900 (4693241) 4693241..4694362 + 1122 WP_002887282.1 cupin domain-containing protein -
LGM00_RS22905 (4694410) 4694410..4694943 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGM00_RS22910 (4694954) 4694954..4695220 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM00_RS22915 (4695323) 4695323..4696756 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM00_RS22920 (4696746) 4696746..4697429 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LGM00_RS22925 (4697601) 4697601..4698986 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
LGM00_RS22930 (4699004) 4699004..4699348 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219705 WP_004178461.1 NZ_CP084871:c4694943-4694410 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219705 NZ_CP116604:1053523-1053780 [Streptococcus sp. HN38]
ATGAAGACTTGTTATAAACTCGTACCAACCAGTCGTTTTATTAAACAGTTGAAAAAGCTGGATAAATTTACTCAAAAGCA
GATTACCAACTACCTGTCCAGTCATGTGACTGACAACCCTAGACAATACGGAAAAGCCTTAACTGCCAATCGTTCTGGCC
AATGGCGCTACCGTATCGGCAATTACAGGGTTATCGTCAGCATCGAAGATGACAAAGTGACAGCTACAAGTCTAAAAATG
AGAAATGGAAGTATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT219705 WP_002887278.1 NZ_CP084871:c4695220-4694954 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219705 NZ_CP116604:1053288-1053539 [Streptococcus sp. HN38]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAACTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTGAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCACTTGAAGATGAGTACGATGCCCGTGTGGCAGACATTGCTTGGGCAG
AATACCAAGAAGACTTGGCGAATGGCATTGAGCCGATTAGCTGGGAGGAAATGTTGGAAGAGTTGGGATTGACGAATGAA
GACTTGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References