Detailed information of TA system TA021970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2051757..2052472
• Replicon: chromosome
• Accession: NC_009053
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APL_RS09715
• Protein ID: WP_005619039.1
• Coordinates: 2051757..2052185 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: APL_RS09720
• Protein ID: WP_005599447.1
• Coordinates: 2052185..2052472 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APL_RS09700	2047341..2049047	-	1707	WP_005609259.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
APL_RS09705	2049150..2049425	-	276	WP_011848662.1	DNA-directed RNA polymerase subunit omega	-
APL_RS09710	2049552..2051633	-	2082	WP_009875323.1	ATP-dependent DNA helicase RecG	-
APL_RS09715	2051757..2052185	-	429	WP_005619039.1	type II toxin-antitoxin system VapC family toxin	Toxin
APL_RS09720	2052185..2052472	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
APL_RS09725	2052678..2054393	+	1716	WP_009875324.1	proline--tRNA ligase	-
APL_RS09730	2054459..2054647	-	189	WP_005602684.1	hypothetical protein	-
APL_RS09735	2054650..2055231	-	582	WP_009875325.1	sigma-70 family RNA polymerase sigma factor	-
APL_RS09740	2055320..2056276	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
APL_RS09745	2056276..2056869	+	594	WP_005599453.1	sulfoxide reductase heme-binding subunit YedZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16508.97 Da
• Isoelectric Point: 8.9724

>T21970 WP_005619039.1 NC_009053:c2052185-2051757 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MFQYLFDTNIISELYKLGSNRMDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 429 bp

>T21970 NC_009053:c2052185-2051757 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAGCAATAGAATGGATACCAATGTACG
ACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACCGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT21970 WP_005599447.1 NC_009053:c2052472-2052185 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT21970 NC_009053:c2052472-2052185 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5