Detailed information of TA system TA021969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1967877..1968505
• Replicon: chromosome
• Accession: NC_009053
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APL_RS09195
• Protein ID: WP_009875032.1
• Coordinates: 1968107..1968505 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: APL_RS09190
• Protein ID: WP_005613300.1
• Coordinates: 1967877..1968107 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APL_RS09185	1965725..1967355	+	1631	Protein_1761	hypothetical protein	-
APL_RS09190	1967877..1968107	+	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
APL_RS09195	1968107..1968505	+	399	WP_009875032.1	tRNA(fMet)-specific endonuclease VapC	Toxin
APL_RS09200	1968640..1969056	-	417	WP_011848651.1	DUF417 family protein	-
APL_RS09205	1969302..1969952	+	651	WP_011848652.1	DUF2202 domain-containing protein	-
APL_RS09210	1970132..1970911	-	780	WP_011848653.1	hypothetical protein	-
APL_RS09215	1970984..1972306	-	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
APL_RS09220	1972361..1972882	-	522	WP_005618211.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1952365..1970911	18546

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15105.39 Da
• Isoelectric Point: 7.4195

>T21969 WP_009875032.1 NC_009053:1968107-1968505 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCISSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T21969 NC_009053:1968107-1968505 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACCCGTCTTTGTATAAGTAGTATCACTGCAGCGGAGCTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTAACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT21969 WP_005613300.1 NC_009053:1967877-1968107 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT21969 NC_009053:1967877-1968107 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGGAAGCATCAGTATTTATGACAAACCGTAGTCAGGCGGTACGATTACCTGCAGAAGTGCGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGCAATGAACGTATTTTGGCTCCAATTAACCAAAGTTGGGATAGCTTCTTTCTGAGTT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGAACTATAA

Structures

Antitoxin

• AlphaFold DB: A3N328