Detailed information of TA system TA219681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3013360..3014948
• Replicon: chromosome
• Accession: NZ_CP084870
• Organism: Klebsiella pneumoniae strain BJ1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LGM01_RS14375
• Protein ID: WP_019705665.1
• Coordinates: 3013626..3014948 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LGM01_RS14370
• Protein ID: WP_002904790.1
• Coordinates: 3013360..3013626 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM01_RS14345 (3008511)	3008511..3009434	+	924	WP_032436334.1	LysR family hpxDE operon transcriptional regulator HpxR	-
LGM01_RS14350 (3009415)	3009415..3010569	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LGM01_RS14355 (3010915)	3010915..3012294	+	1380	WP_032446036.1	nucleobase:cation symporter-2 family protein	-
LGM01_RS14360 (3012272)	3012272..3012772	+	501	WP_032446037.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LGM01_RS14365 (3012769)	3012769..3013095	+	327	WP_002904788.1	hydroxyisourate hydrolase	-
LGM01_RS14370 (3013360)	3013360..3013626	+	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LGM01_RS14375 (3013626)	3013626..3014948	+	1323	WP_019705665.1	type II toxin-antitoxin system HipA family toxin	Toxin
LGM01_RS14380 (3014965)	3014965..3016257	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LGM01_RS14385 (3016399)	3016399..3017403	-	1005	WP_032446038.1	fimbrial protein	-
LGM01_RS14390 (3017391)	3017391..3019892	-	2502	WP_032446039.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5358

>T219681 WP_019705665.1 NZ_CP084870:3013626-3014948 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T219681 NZ_CP116575:2770990-2771133 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT219681 WP_002904790.1 NZ_CP084870:3013360-3013626 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT219681 NZ_CP116575:c2771250-2771066 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8