Detailed information of TA system TA021968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1905189..1906846
• Replicon: chromosome
• Accession: NC_009053
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A3N2X8
• Locus tag: APL_RS08920
• Protein ID: WP_011848633.1
• Coordinates: 1905515..1906846 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A3N2X7
• Locus tag: APL_RS08915
• Protein ID: WP_005613259.1
• Coordinates: 1905189..1905518 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APL_RS08895	1902519..1903136	+	618	WP_009874973.1	dimethylsulfoxide reductase subunit B	-
APL_RS08900	1903138..1903971	+	834	WP_009874972.1	dimethyl sulfoxide reductase anchor subunit	-
APL_RS08905	1904027..1904632	+	606	WP_009874971.1	Tat proofreading chaperone DmsD	-
APL_RS08910	1904636..1905145	+	510	WP_009874970.1	ferredoxin-type protein NapF	-
APL_RS08915	1905189..1905518	-	330	WP_005613259.1	helix-turn-helix domain-containing protein	Antitoxin
APL_RS08920	1905515..1906846	-	1332	WP_011848633.1	type II toxin-antitoxin system HipA family toxin	Toxin
APL_RS08925	1907178..1908869	-	1692	WP_011848634.1	AIPR family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-371)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51131.07 Da
• Isoelectric Point: 5.0347

>T21968 WP_011848633.1 NC_009053:c1906846-1905515 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MSDISVKNKKLTLQTYVDNLWRDMAELYFNAQYELESMEYLTDYAVDFFGQANARSISINYPVNVFIDHADKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAVPEIQSERYFPLNVVQNRQSDFLEYANQTGAMVGG
ATGAGGEAPKLLLKCQDKQVWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
ERYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNIISASQFSNDFDNRHFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNRIWLSPIYDFAPMRADPEGIIRTTTWAKNGDNPQEFAGEYRFDKIADGLSDLIEPDILLRELR
MLATQLVDLKSRLMKRGVPKSILDFPAIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

>T21968 NC_009053:c1906846-1905515 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGAGTGATATATCAGTCAAAAATAAAAAATTAACATTGCAGACCTATGTTGATAATCTATGGCGAGATATGGCGGAGCT
TTATTTCAATGCTCAGTATGAATTAGAGAGCATGGAATATTTAACGGACTATGCCGTCGATTTTTTTGGGCAAGCGAATG
CCAGATCCATATCAATTAATTATCCTGTAAATGTGTTTATTGATCATGCCGATAAGCTGATGACTACACTTGCCGATATT
ATTCCTGCCGGAGCGAGTCGGCGTTATTGGCTGGAAGCTCTTGATATCAAGCACCTATCTATTTCTCATCAAAATTACTT
ATTGCTAAAAAATGGCGCAATATCCCCTATCGGCAATGTCCGAGTTAAAGAGGCTGTGCCAGAAATACAAAGTGAGCGTT
ATTTTCCATTAAATGTGGTACAAAACAGGCAGAGTGATTTTTTAGAATATGCAAATCAGACAGGAGCTATGGTAGGCGGA
GCGACTGGGGCTGGGGGAGAAGCACCAAAGCTATTACTTAAATGCCAAGATAAACAAGTTTGGATTGATAATTTACAATT
AGGTCTAGATTGCGATACGGCTTATTTAGTGAAATATCCTCGTGGGAATTATTCAGAGTTGGACTGTGATATTTTAAGAG
CTGAATATCATTATTATCATGAGCTGCGTGCATTAGGGTTTTCAACTATAGATACTGATCTAATGAAATTAGAAGAGGGG
GAACGTTATCCGTCTTTATGGCTACCTAGATTCGATATGAAGTTCAAAGATGGAAAATGGGAGCGATATGCTATGGAATC
GGTTTATTCTATGTTACAAAAATATGGTGGAAATCTTGATCATGAGCAGACTATTCGCAACTTACTCAATATTATTTCAG
CTTCGCAATTTTCTAATGATTTTGATAACCGGCATTTTGTTATTGAATGGGTAAAGCGAGATTTGTTGAATATTGCTTTT
GGAAATAGTGATAATCATGGCAGAAATACTGCATTTTTACGTGATGATAATCGTATTTGGCTATCCCCTATCTATGATTT
TGCTCCGATGCGGGCAGATCCGGAAGGCATTATCAGAACGACCACTTGGGCTAAAAATGGAGATAACCCTCAAGAGTTCG
CCGGAGAATATCGCTTTGATAAAATAGCTGACGGTTTATCTGACCTTATTGAACCAGATATATTGTTAAGAGAATTAAGA
ATGCTTGCTACCCAATTAGTAGATTTAAAATCTAGACTTATGAAGCGAGGTGTTCCGAAAAGTATTTTAGATTTTCCCGC
CATTGGTTTTGATTACTTACCTGAGAAGTTACAACGTTGGGGGCTGGTATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12320.45 Da
• Isoelectric Point: 10.8292

>AT21968 WP_005613259.1 NC_009053:c1905518-1905189 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MTIQHKNASPTRSKVNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETINQVFKPMGLKLGLMPISKDLMASFLK

Nucleotide

• Length: 330 bp

>AT21968 NC_009053:c1905518-1905189 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGACGATTCAACATAAAAATGCCTCACCGACGCGATCTAAAGTGAATGGCATTGAACGACAATTAATCCGTGAAACGAT
TATAGTACAGCTCTTAAAAGGTGAGATTAGTCAAGGACAAGCACTACGTCGATTACGGGTAGAAGCATTAGGAATAAACC
AGCAAGATTATCTAAAATTAGCGAAAGTTTCTCGGCAAACGCTTTCTAATATCGAAAATGATAAAGGAAACTATAGTATA
GAGACAATTAATCAGGTATTTAAACCGATGGGACTAAAGCTAGGTCTTATGCCGATCTCAAAAGACTTGATGGCTTCCTT
TCTTAAATAA

Structures

Toxin

• AlphaFold DB: A3N2X8

Antitoxin

• AlphaFold DB: A3N2X7