Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1103950..1104760 Replicon chromosome
Accession NZ_CP084870
Organism Klebsiella pneumoniae strain BJ1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGM01_RS05340 Protein ID WP_004178461.1
Coordinates 1104227..1104760 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LGM01_RS05335 Protein ID WP_002887278.1
Coordinates 1103950..1104216 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM01_RS05315 (1099825) 1099825..1100169 - 345 WP_025987660.1 cation efflux system protein CusF -
LGM01_RS05320 (1100187) 1100187..1101569 - 1383 WP_032446519.1 efflux transporter outer membrane subunit -
LGM01_RS05325 (1101741) 1101741..1102424 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
LGM01_RS05330 (1102414) 1102414..1103847 + 1434 WP_032446518.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM01_RS05335 (1103950) 1103950..1104216 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM01_RS05340 (1104227) 1104227..1104760 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGM01_RS05345 (1104808) 1104808..1105929 - 1122 WP_009309849.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219678 WP_004178461.1 NZ_CP084870:1104227-1104760 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219678 NZ_CP116575:1846375-1846719 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAACGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTCTCCCATTCGGATTATATTGAGTTATATAAAATTCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCAGATTAT
CATATGTTTAAATGTCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAGGAAACTTATTTAAATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT219678 WP_002887278.1 NZ_CP084870:1103950-1104216 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219678 NZ_CP116575:1846025-1846357 [Enterococcus faecalis]
ATGAATACTTACGAAATAATAAAAGACTTAGCTAAAGAAAAAGGAATTTCTATTAGACAATTGGAAATAAATTTTGGCTA
CTCTAATGGATATATCGGTAGTTGGAAAAGACAAACACCCAATTCTAATGAATTAAGTCGCTTAGCAGACTACTTTGATG
TATCTGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAAATCTGATGATTTAGATGATGTA
CTGGATAACGTCATGAGTTTTGACGGTGAACCACTTGATGATCATGACAGAGAAGTTATTCGTGCTTACTTAAAAGGAAG
ATTCGGAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References