Detailed information of TA system TA219676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 845888..847569
• Replicon: chromosome
• Accession: NZ_CP084870
• Organism: Klebsiella pneumoniae strain BJ1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: LGM01_RS04080
• Protein ID: WP_004146663.1
• Coordinates: 846652..847569 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LGM01_RS04075
• Protein ID: WP_032446592.1
• Coordinates: 845888..846646 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM01_RS04060 (841738)	841738..842424	-	687	WP_004151733.1	tetratricopeptide repeat protein	-
LGM01_RS04065 (842654)	842654..844801	-	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
LGM01_RS04070 (845074)	845074..845802	+	729	WP_002885152.1	response regulator transcription factor	-
LGM01_RS04075 (845888)	845888..846646	+	759	WP_032446592.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LGM01_RS04080 (846652)	846652..847569	+	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LGM01_RS04085 (847694)	847694..848684	-	991	Protein_782	carbohydrate kinase family protein	-
LGM01_RS04090 (848759)	848759..849700	-	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LGM01_RS04095 (849731)	849731..850729	-	999	WP_031590230.1	ribose ABC transporter permease	-
LGM01_RS04100 (850726)	850726..852246	-	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T219676 WP_004146663.1 NZ_CP084870:846652-847569 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T219676 NZ_CP116575:414457-414765 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28566.94 Da
• Isoelectric Point: 10.1296

>AT219676 WP_032446592.1 NZ_CP084870:845888-846646 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDAFACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRFRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT219676 NZ_CP116575:414183-414455 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: J2XCI1