Detailed information of TA system TA219672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 170631..171301
• Replicon: plasmid pKp_SA1_1
• Accession: NZ_CP084869
• Organism: Klebsiella pneumoniae strain SA1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LGM08_RS26735
• Protein ID: WP_004213072.1
• Coordinates: 170631..171074 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LGM08_RS26740
• Protein ID: WP_004213073.1
• Coordinates: 171071..171301 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM08_RS26700 (LGM08_26700)	166037..166312	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LGM08_RS26705 (LGM08_26705)	166375..166866	+	492	WP_011251327.1	DM13 domain-containing protein	-
LGM08_RS26710 (LGM08_26710)	166915..167835	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LGM08_RS26715 (LGM08_26715)	167926..168329	+	404	Protein_187	GAF domain-containing protein	-
LGM08_RS26720 (LGM08_26720)	168847..169487	-	641	Protein_188	mucoid phenotype regulator RmpA2	-
LGM08_RS26725 (LGM08_26725)	169904..170208	+	305	Protein_189	transposase	-
LGM08_RS26730 (LGM08_26730)	170231..170482	-	252	WP_186987481.1	hypothetical protein	-
LGM08_RS26735 (LGM08_26735)	170631..171074	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LGM08_RS26740 (LGM08_26740)	171071..171301	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LGM08_RS26745 (LGM08_26745)	171909..173042	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LGM08_RS26750 (LGM08_26750)	173058..173351	+	294	WP_004213076.1	hypothetical protein	-
LGM08_RS26755 (LGM08_26755)	173341..173547	-	207	WP_004213077.1	hypothetical protein	-
LGM08_RS26760 (LGM08_26760)	173899..174189	+	291	WP_004213078.1	hypothetical protein	-
LGM08_RS26765 (LGM08_26765)	174179..175078	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2	1..239249	239249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T219672 WP_004213072.1 NZ_CP084869:c171074-170631 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T219672 NZ_CP116571:2828297-2828440 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT219672 WP_004213073.1 NZ_CP084869:c171301-171071 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT219672 NZ_CP116571:c2828200-2827999 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620