Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 70168..70904 Replicon plasmid pKp_SA1_1
Accession NZ_CP084869
Organism Klebsiella pneumoniae strain SA1

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LGM08_RS26150 Protein ID WP_004098919.1
Coordinates 70422..70904 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LGM08_RS26145 Protein ID WP_004213599.1
Coordinates 70168..70434 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM08_RS26120 (LGM08_26120) 65400..65957 + 558 WP_004213590.1 recombinase family protein -
LGM08_RS26125 (LGM08_26125) 65960..68929 + 2970 WP_004213592.1 Tn3 family transposase -
LGM08_RS26130 (LGM08_26130) 68971..69465 + 495 WP_004213594.1 hypothetical protein -
LGM08_RS26135 (LGM08_26135) 69526..69729 + 204 WP_004213596.1 HHA domain-containing protein -
LGM08_RS26140 (LGM08_26140) 69743..69973 + 231 WP_004213598.1 hypothetical protein -
LGM08_RS26145 (LGM08_26145) 70168..70434 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LGM08_RS26150 (LGM08_26150) 70422..70904 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LGM08_RS26155 (LGM08_26155) 71325..72552 + 1228 Protein_75 IS3 family transposase -
LGM08_RS26160 (LGM08_26160) 72548..72943 - 396 Protein_76 IS3 family transposase -
LGM08_RS26165 (LGM08_26165) 73118..74140 - 1023 WP_004214536.1 porphobilinogen synthase -
LGM08_RS26170 (LGM08_26170) 74204..75550 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 1..239249 239249
- inside IScluster/Tn - - 65400..72552 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T219671 WP_004098919.1 NZ_CP084869:70422-70904 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T219671 NZ_CP116571:2827926-2828066 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT219671 WP_004213599.1 NZ_CP084869:70168-70434 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT219671 NZ_CP116571:c2828200-2827999 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References