Detailed information of TA system TA021967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1752542..1753273
• Replicon: chromosome
• Accession: NC_009053
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A3N2I6
• Locus tag: APL_RS08125
• Protein ID: WP_009875245.1
• Coordinates: 1752542..1753009 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: APL_RS08130
• Protein ID: WP_005619269.1
• Coordinates: 1753013..1753273 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APL_RS08100	1747900..1748271	-	372	WP_005598830.1	CrcB family protein	-
APL_RS08105	1748274..1749338	-	1065	WP_011848610.1	MFS transporter	-
APL_RS08110	1749433..1750542	+	1110	WP_009875243.1	anhydro-N-acetylmuramic acid kinase	-
APL_RS08115	1750596..1751510	+	915	WP_009875244.1	N-acetylmuramic acid 6-phosphate etherase	-
APL_RS08120	1751577..1752458	-	882	WP_005608878.1	50S ribosomal protein L11 methyltransferase	-
APL_RS08125	1752542..1753009	-	468	WP_009875245.1	GNAT family N-acetyltransferase	Toxin
APL_RS08130	1753013..1753273	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
APL_RS08135	1753394..1754854	-	1461	WP_009875246.1	metalloprotease TldD	-
APL_RS08140	1754984..1756243	+	1260	WP_005608887.1	tRNA lysidine(34) synthetase TilS	-
APL_RS11065	1756270..1756560	-	291	Protein_1570	DUF5389 family protein	-
APL_RS08145	1756562..1757455	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17227.20 Da
• Isoelectric Point: 9.5125

>T21967 WP_009875245.1 NC_009053:c1753009-1752542 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVRDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEIVLMLKL

Nucleotide

• Length: 468 bp

>T21967 NC_009053:c1753009-1752542 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGCGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGCTCAGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAATCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCCGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATAGTTTTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT21967 WP_005619269.1 NC_009053:c1753273-1753013 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT21967 NC_009053:c1753273-1753013 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: A3N2I6

Antitoxin

• AlphaFold DB: A3N2I7