Detailed information of TA system TA021966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1554593..1555238
• Replicon: chromosome
• Accession: NC_009053
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: APL_RS07145
• Protein ID: WP_005619674.1
• Coordinates: 1554867..1555238 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: APL_RS07140
• Protein ID: WP_005598477.1
• Coordinates: 1554593..1554886 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APL_RS07110	1549925..1550608	-	684	WP_005605188.1	arginine ABC transporter permease ArtM	-
APL_RS07115	1550608..1551279	-	672	WP_011848573.1	arginine ABC transporter permease ArtQ	-
APL_RS07120	1551285..1552019	-	735	WP_009874916.1	transporter substrate-binding domain-containing protein	-
APL_RS07125	1552024..1552758	-	735	WP_011848575.1	arginine ABC transporter ATP-binding protein ArtP	-
APL_RS07130	1552983..1553480	-	498	WP_005601953.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
APL_RS07135	1553553..1554515	+	963	WP_011848576.1	calcium/sodium antiporter	-
APL_RS07140	1554593..1554886	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
APL_RS07145	1554867..1555238	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APL_RS07150	1555432..1557183	+	1752	WP_005601957.1	protein-disulfide reductase DsbD	-
APL_RS07155	1557241..1557810	+	570	WP_005601959.1	elongation factor P hydroxylase	-
APL_RS07160	1557854..1558708	-	855	WP_009874917.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T21966 WP_005619674.1 NC_009053:c1555238-1554867 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T21966 NC_009053:c1555238-1554867 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT21966 WP_005598477.1 NC_009053:c1554886-1554593 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT21966 NC_009053:c1554886-1554593 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATTTCTCAACGAAAATTGGAGAAATTAAGCGGCG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206