Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 967599..968409 Replicon chromosome
Accession NZ_CP084868
Organism Klebsiella pneumoniae strain SA1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LGM08_RS04515 Protein ID WP_004178461.1
Coordinates 967876..968409 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LGM08_RS04510 Protein ID WP_002887278.1
Coordinates 967599..967865 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM08_RS04490 (963471) 963471..963815 - 345 WP_004214146.1 cation efflux system protein CusF -
LGM08_RS04495 (963833) 963833..965218 - 1386 WP_004214145.1 efflux transporter outer membrane subunit -
LGM08_RS04500 (965390) 965390..966073 + 684 WP_004214143.1 copper response regulator transcription factor CusR -
LGM08_RS04505 (966063) 966063..967496 + 1434 WP_004214141.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM08_RS04510 (967599) 967599..967865 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM08_RS04515 (967876) 967876..968409 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LGM08_RS04520 (968457) 968457..969578 - 1122 WP_004214138.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T219657 WP_004178461.1 NZ_CP084868:967876-968409 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219657 NZ_CP116569:2630883-2631026 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT219657 WP_002887278.1 NZ_CP084868:967599-967865 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219657 NZ_CP116569:c2631160-2630959 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References