Detailed information of TA system TA219656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 912970..914307
• Replicon: chromosome
• Accession: NZ_CP084868
• Organism: Klebsiella pneumoniae strain SA1

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: LGM08_RS04265
• Protein ID: WP_032424556.1
• Coordinates: 912970..913905 (-)
• Length: 312 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: LGM08_RS04270
• Protein ID: WP_023302769.1
• Coordinates: 913912..914307 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM08_RS04250 (909191)	909191..910870	+	1680	WP_004212561.1	tyrosine-type recombinase/integrase	-
LGM08_RS04255 (911391)	911391..912083	+	693	WP_223272586.1	RES family NAD+ phosphorylase	-
LGM08_RS04260 (912273)	912273..912962	+	690	WP_023302767.1	abortive infection system antitoxin AbiGi family protein	-
LGM08_RS04265 (912970)	912970..913905	-	936	WP_032424556.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LGM08_RS04270 (913912)	913912..914307	-	396	WP_023302769.1	DUF6088 family protein	Antitoxin
LGM08_RS04275 (914562)	914562..914828	+	267	WP_023302770.1	hypothetical protein	-
LGM08_RS04280 (914834)	914834..915298	-	465	WP_022615700.1	GNAT family N-acetyltransferase	-
LGM08_RS04285 (915470)	915470..917065	-	1596	WP_023302771.1	MobH family relaxase	-
LGM08_RS04290 (917239)	917239..917610	-	372	WP_022615702.1	hypothetical protein	-
LGM08_RS04295 (917803)	917803..918708	-	906	WP_022615703.1	zincin-like metallopeptidase domain-containing protein	-
LGM08_RS04300 (918773)	918773..919063	-	291	WP_022615704.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-298)

Antitoxin

• Protein of unknown function DUF6088 (7-109)

Sequences

Toxin

Protein

• Length: 312 a.a.
• Molecular weight: 35389.66 Da
• Isoelectric Point: 5.6165

>T219656 WP_032424556.1 NZ_CP084868:c913905-912970 [Klebsiella pneumoniae]
MLFSDVTDALGIESGAIVEKDHYIVELLHLLKPLTFDTHQLLFAGGTALAKSGIVLNRMSEDVDIKLVPTDTFLSLSRTQ
RKGIRKEIVQTISHAITATDMFSFDDAHPKVTRDEYRYNDIPVRYPQRVAQAPCLRPFIQLELMEANLLEAPEFRDIHTL
VTTLTGKAPVISAFPCATIISTQAEKLVAMLRRTAAVARNIERQDDASLVRHIYDTYCIVQAGNVDMCLLTDFFKQTIEQ
DIQRYGNQYPQFCVSPNDELKMGLDELEHNPLYKTRYQQFVAPMVYGARKVSWDEAYASFRQMALIILDTY

Nucleotide

• Length: 936 bp

>T219656 NZ_CP116569:c2624477-2624136 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14800.08 Da
• Isoelectric Point: 11.1078

>AT219656 WP_023302769.1 NZ_CP084868:c914307-913912 [Klebsiella pneumoniae]
MTIKTRIQSRLKRSKRYVFTRDDFKDIAGYDQIGRVLRDLLKEGQLLKVGYGVYTKARRNSITGKVMPAAPRGSSAVIVE
TLERLKVRYRYEGATQTYNSGESTQIPVSPEIKTSPRFKRALSVGNSKLNG

Nucleotide

• Length: 396 bp

>AT219656 NZ_CP116569:c2624707-2624477 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA