Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 374371..374996 Replicon chromosome
Accession NZ_CP084868
Organism Klebsiella pneumoniae strain SA1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGM08_RS01725 Protein ID WP_002882817.1
Coordinates 374613..374996 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM08_RS01720 Protein ID WP_004150355.1
Coordinates 374371..374613 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM08_RS01695 (370361) 370361..370960 + 600 WP_004151865.1 glucose-1-phosphatase -
LGM08_RS01700 (370954) 370954..371814 + 861 WP_004146232.1 virulence factor BrkB family protein -
LGM08_RS01705 (371811) 371811..372248 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGM08_RS01710 (372293) 372293..373234 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM08_RS01715 (373249) 373249..374166 - 918 WP_004213381.1 alpha/beta hydrolase -
LGM08_RS01720 (374371) 374371..374613 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM08_RS01725 (374613) 374613..374996 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM08_RS01730 (375170) 375170..376099 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGM08_RS01735 (376096) 376096..376731 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGM08_RS01740 (376728) 376728..377630 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219653 WP_002882817.1 NZ_CP084868:374613-374996 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219653 NZ_CP116569:471456-471572 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219653 WP_004150355.1 NZ_CP084868:374371-374613 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219653 NZ_CP116569:c471688-471483 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References