Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 154982..155652 Replicon plasmid pKp_SA12_1
Accession NZ_CP084867
Organism Klebsiella pneumoniae strain SA12

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag LGM09_RS27085 Protein ID WP_004213072.1
Coordinates 154982..155425 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag LGM09_RS27090 Protein ID WP_004213073.1
Coordinates 155422..155652 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM09_RS27050 (LGM09_27050) 150388..150663 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
LGM09_RS27055 (LGM09_27055) 150726..151217 + 492 WP_011251327.1 DM13 domain-containing protein -
LGM09_RS27060 (LGM09_27060) 151266..152186 + 921 WP_004213934.1 DUF1471 domain-containing protein -
LGM09_RS27065 (LGM09_27065) 152277..152680 + 404 Protein_166 GAF domain-containing protein -
LGM09_RS27070 (LGM09_27070) 153198..153838 - 641 Protein_167 mucoid phenotype regulator RmpA2 -
LGM09_RS27075 (LGM09_27075) 154255..154559 + 305 Protein_168 transposase -
LGM09_RS27080 (LGM09_27080) 154582..154833 - 252 WP_186987481.1 hypothetical protein -
LGM09_RS27085 (LGM09_27085) 154982..155425 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM09_RS27090 (LGM09_27090) 155422..155652 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LGM09_RS27095 (LGM09_27095) 156260..157393 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
LGM09_RS27100 (LGM09_27100) 157409..157702 + 294 WP_226541787.1 korC -
LGM09_RS27105 (LGM09_27105) 157692..157898 - 207 WP_004213077.1 hypothetical protein -
LGM09_RS27110 (LGM09_27110) 158250..158540 + 291 WP_004213078.1 hypothetical protein -
LGM09_RS27115 (LGM09_27115) 158530..159429 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA 1..230142 230142


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T219652 WP_004213072.1 NZ_CP084867:c155425-154982 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T219652 NZ_CP116569:c308779-308684 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT219652 WP_004213073.1 NZ_CP084867:c155652-155422 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT219652 NZ_CP116569:308585-308649 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References