Detailed information of TA system TA021965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1468514..1469022
• Replicon: chromosome
• Accession: NC_009053
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: APL_RS06685
• Protein ID: WP_009875288.1
• Coordinates: 1468762..1469022 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A3N1S6
• Locus tag: APL_RS06680
• Protein ID: WP_005605062.1
• Coordinates: 1468514..1468765 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APL_RS06640	1464050..1464598	-	549	WP_011848557.1	type IV pilus biogenesis/stability protein PilW	-
APL_RS06645	1464652..1465833	-	1182	WP_009875290.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
APL_RS06670	1466737..1468176	+	1440	WP_011848558.1	glutamate--tRNA ligase	-
APL_RS06680	1468514..1468765	+	252	WP_005605062.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
APL_RS06685	1468762..1469022	+	261	WP_009875288.1	Txe/YoeB family addiction module toxin	Toxin
APL_RS06690	1469179..1469346	-	168	WP_009875287.1	Trm112 family protein	-
APL_RS06695	1469348..1470328	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
APL_RS06700	1470422..1471681	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
APL_RS06705	1471681..1472271	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
APL_RS06710	1472393..1472785	+	393	WP_005620423.1	SufE family protein	-
APL_RS06725	1473144..1473917	-	774	WP_009875285.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10452.00 Da
• Isoelectric Point: 8.0109

>T21965 WP_009875288.1 NC_009053:1468762-1469022 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKPEPLKFNLSGFWSRRINEEHRLIYTVEDETILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T21965 NC_009053:1468762-1469022 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGTCTGATTAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAGCCTGAACCGCTAAAATTCAATCTTTCGG
GCTTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACACCGTGGAAGATGAAACAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9320.62 Da
• Isoelectric Point: 4.5659

>AT21965 WP_005605062.1 NC_009053:1468514-1468765 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYSSLEETAYLMRSPANAQRLLDSMAELKQGKGIERE
LIE

Nucleotide

• Length: 252 bp

>AT21965 NC_009053:1468514-1468765 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACAATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATAGTTCACTTGAAGAAACCGCCTATT
TAATGCGTTCCCCGGCTAACGCTCAAAGATTACTAGACAGCATGGCAGAATTGAAGCAAGGGAAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Antitoxin

• AlphaFold DB: A3N1S6