Detailed information of TA system TA219644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 2736436..2737346
• Replicon: chromosome
• Accession: NZ_CP084866
• Organism: Klebsiella pneumoniae strain SA12

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LGM09_RS13385
• Protein ID: WP_004026354.1
• Coordinates: 2736436..2736906 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LGM09_RS13390
• Protein ID: WP_004026357.1
• Coordinates: 2736903..2737346 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM09_RS13345 (2731520)	2731520..2732122	-	603	WP_011251277.1	HAD domain-containing protein	-
LGM09_RS13350 (2732125)	2732125..2732640	-	516	WP_041937820.1	hypothetical protein	-
LGM09_RS13355 (2732748)	2732748..2733323	-	576	WP_074424927.1	hypothetical protein	-
LGM09_RS13360 (2733440)	2733440..2734396	-	957	WP_011251280.1	DsbA family protein	-
LGM09_RS13365 (2734456)	2734456..2734797	-	342	WP_011251281.1	hypothetical protein	-
LGM09_RS13370 (2734811)	2734811..2735122	-	312	WP_011251282.1	hypothetical protein	-
LGM09_RS13375 (2735139)	2735139..2735588	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LGM09_RS13385 (2736436)	2736436..2736906	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LGM09_RS13390 (2736903)	2736903..2737346	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LGM09_RS13395 (2737447)	2737447..2737950	-	504	Protein_2619	DUF4113 domain-containing protein	-
LGM09_RS13400 (2738048)	2738048..2738269	-	222	WP_004213643.1	DUF2188 domain-containing protein	-
LGM09_RS13405 (2738450)	2738450..2738569	-	120	Protein_2621	Fe3+-citrate ABC transporter substrate-binding protein	-
LGM09_RS13410 (2738644)	2738644..2740770	-	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
LGM09_RS13415 (2740857)	2740857..2741810	-	954	Protein_2623	fec operon regulator FecR	-
LGM09_RS13420 (2741807)	2741807..2742328	-	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	iroB / iroC / iroD / iroN / rmpA	2723278..2773042	49764
	-	flank	IS/Tn	-	-	2735590..2736093	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T219644 WP_004026354.1 NZ_CP084866:c2736906-2736436 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T219644 NZ_CP116566:c2534998-2534897 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT219644 WP_004026357.1 NZ_CP084866:c2737346-2736903 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT219644 NZ_CP116566:2534776-2534960 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAG
TTTTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAA
AATCAGTAACGCAACAAGGGTTGCA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9