Detailed information of TA system TA021964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 760115..760765
• Replicon: chromosome
• Accession: NC_009053
• Organism: Actinobacillus pleuropneumoniae serovar 5b str. L20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: APL_RS03475
• Protein ID: WP_005614926.1
• Coordinates: 760115..760504 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: APL_RS03480
• Protein ID: WP_005604117.1
• Coordinates: 760505..760765 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APL_RS03460	755692..756882	+	1191	WP_193327847.1	aspartate/tyrosine/aromatic aminotransferase	-
APL_RS03465	757061..758347	-	1287	WP_009874699.1	Xaa-Pro aminopeptidase	-
APL_RS03470	758756..759979	-	1224	WP_009874700.1	ATP-binding protein	-
APL_RS03475	760115..760504	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
APL_RS03480	760505..760765	-	261	WP_005604117.1	hypothetical protein	Antitoxin
APL_RS03485	760899..762920	-	2022	WP_009874701.1	excinuclease ABC subunit B	-
APL_RS03490	763097..763909	+	813	WP_005607533.1	EfeM/EfeO family lipoprotein	-
APL_RS03495	763921..765114	+	1194	WP_009874702.1	deferrochelatase/peroxidase EfeB	-
APL_RS03500	765125..765745	+	621	WP_009874703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T21964 WP_005614926.1 NC_009053:c760504-760115 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T21964 NC_009053:c760504-760115 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT21964 WP_005604117.1 NC_009053:c760765-760505 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT21964 NC_009053:c760765-760505 [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030