Detailed information of TA system TA219632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 81175..81911
• Replicon: plasmid pKp_SB61067_1
• Accession: NZ_CP084861
• Organism: Klebsiella pneumoniae strain SB1067

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LGM10_RS25985
• Protein ID: WP_136046327.1
• Coordinates: 81175..81657 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LGM10_RS25990
• Protein ID: WP_003026799.1
• Coordinates: 81645..81911 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM10_RS25970 (LGM10_25970)	77508..79030	+	1523	Protein_81	IS3 family transposase	-
LGM10_RS25975 (LGM10_25975)	79178..79573	-	396	WP_021312679.1	helix-turn-helix domain-containing protein	-
LGM10_RS25980 (LGM10_25980)	79622..80968	-	1347	WP_077263552.1	ISNCY family transposase	-
LGM10_RS25985 (LGM10_25985)	81175..81657	-	483	WP_136046327.1	GNAT family N-acetyltransferase	Toxin
LGM10_RS25990 (LGM10_25990)	81645..81911	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LGM10_RS25995 (LGM10_25995)	83023..83832	-	810	WP_023329017.1	N-formylglutamate deformylase	-
LGM10_RS26000 (LGM10_26000)	83825..85033	-	1209	WP_057072740.1	imidazolonepropionase	-
LGM10_RS26005 (LGM10_26005)	85045..85458	-	414	WP_226616206.1	cytosine permease	-
LGM10_RS26010 (LGM10_26010)	85524..86492	-	969	WP_004099053.1	IS5-like element IS903B family transposase	-
LGM10_RS26015 (LGM10_26015)	86600..86803	-	204	WP_004150739.1	HHA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..226445	226445
	-	inside	IScluster/Tn	-	-	58744..86492	27748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (73-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17204.84 Da
• Isoelectric Point: 8.7375

>T219632 WP_136046327.1 NZ_CP084861:c81657-81175 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFSSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T219632 NZ_CP116564:c2584727-2584626 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT219632 WP_003026799.1 NZ_CP084861:c81911-81645 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT219632 NZ_CP116564:2584504-2584689 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAATTT
TTGTTACTATTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAACGCAACAAGGGTTGCA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66