Detailed information of TA system TA219626

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4075811..4076517
• Replicon: chromosome
• Accession: NZ_CP084860
• Organism: Klebsiella pneumoniae strain SB1067

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: LGM10_RS20175
• Protein ID: WP_024622903.1
• Coordinates: 4076149..4076517 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A485WAM6
• Locus tag: LGM10_RS20170
• Protein ID: WP_023302278.1
• Coordinates: 4075811..4076128 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM10_RS20125 (4070952)	4070952..4071776	+	825	WP_023302271.1	DUF932 domain-containing protein	-
LGM10_RS20130 (4071985)	4071985..4072695	+	711	WP_023302272.1	DeoR family transcriptional regulator	-
LGM10_RS20135 (4072721)	4072721..4073257	+	537	WP_023302273.1	DUF4339 domain-containing protein	-
LGM10_RS20140 (4073299)	4073299..4073736	+	438	WP_023301392.1	hypothetical protein	-
LGM10_RS20145 (4073803)	4073803..4074213	+	411	WP_023302274.1	hypothetical protein	-
LGM10_RS20150 (4074291)	4074291..4074527	+	237	WP_032410024.1	DUF905 domain-containing protein	-
LGM10_RS20155 (4074614)	4074614..4075072	+	459	WP_023302275.1	antirestriction protein	-
LGM10_RS20160 (4075081)	4075081..4075563	+	483	WP_023302276.1	RadC family protein	-
LGM10_RS20165 (4075572)	4075572..4075793	+	222	WP_023302277.1	DUF987 domain-containing protein	-
LGM10_RS20170 (4075811)	4075811..4076128	+	318	WP_023302278.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LGM10_RS20175 (4076149)	4076149..4076517	+	369	WP_024622903.1	TA system toxin CbtA family protein	Toxin
LGM10_RS20180 (4076514)	4076514..4076855	+	342	WP_024622904.1	hypothetical protein	-
LGM10_RS20185 (4076978)	4076978..4079623	-	2646	WP_024622905.1	LuxR C-terminal-related transcriptional regulator	-
LGM10_RS20190 (4079997)	4079997..4080956	+	960	WP_023302280.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.82 Da
• Isoelectric Point: 7.2897

>T219626 WP_024622903.1 NZ_CP084860:4076149-4076517 [Klebsiella pneumoniae]
MKNLPATISQAAKPCLSPVAVWQMLLTHLLEQHYGLMLSDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRYSYREVSNIVLSRSRI

Nucleotide

• Length: 369 bp

>T219626 NZ_CP116562:21794-22657 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11860.67 Da
• Isoelectric Point: 6.4629

>AT219626 WP_023302278.1 NZ_CP084860:4075811-4076128 [Klebsiella pneumoniae]
MSNPTWGLLRDITPCLGARLVQEGNRLYYLVDRASITGKFSDAEILKLDVVFPHFISQMESMLTIGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT219626 NZ_CP116562:21520-21792 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A485WAM6