Detailed information of TA system TA219624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2569686..2571274
• Replicon: chromosome
• Accession: NZ_CP084860
• Organism: Klebsiella pneumoniae strain SB1067

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: LGM10_RS12755
• Protein ID: WP_023301940.1
• Coordinates: 2569686..2571008 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LGM10_RS12760
• Protein ID: WP_024622638.1
• Coordinates: 2571008..2571274 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM10_RS12740 (2564742)	2564742..2567243	+	2502	WP_023301938.1	fimbria/pilus outer membrane usher protein	-
LGM10_RS12745 (2567231)	2567231..2568235	+	1005	WP_023301939.1	fimbrial protein	-
LGM10_RS12750 (2568377)	2568377..2569669	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LGM10_RS12755 (2569686)	2569686..2571008	-	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
LGM10_RS12760 (2571008)	2571008..2571274	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LGM10_RS12765 (2571539)	2571539..2571865	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
LGM10_RS12770 (2571862)	2571862..2572362	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LGM10_RS12775 (2572340)	2572340..2573719	-	1380	WP_023301941.1	nucleobase:cation symporter-2 family protein	-
LGM10_RS12780 (2574065)	2574065..2575219	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LGM10_RS12785 (2575200)	2575200..2576123	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T219624 WP_023301940.1 NZ_CP084860:c2571008-2569686 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T219624 NZ_CP116561:c2723130-2722789 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACGCGATATACGTTGCCCGATGACATAGAAACTCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT219624 WP_024622638.1 NZ_CP084860:c2571274-2571008 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT219624 NZ_CP116561:c2723360-2723130 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A486S9S0