Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4694862..4695672 Replicon chromosome
Accession NZ_CP084859
Organism Klebsiella pneumoniae strain SB1139

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag LGM11_RS23115 Protein ID WP_023279404.1
Coordinates 4694862..4695395 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LGM11_RS23120 Protein ID WP_002887278.1
Coordinates 4695406..4695672 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM11_RS23110 (4693693) 4693693..4694814 + 1122 WP_002887282.1 cupin domain-containing protein -
LGM11_RS23115 (4694862) 4694862..4695395 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
LGM11_RS23120 (4695406) 4695406..4695672 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LGM11_RS23125 (4695775) 4695775..4697208 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
LGM11_RS23130 (4697198) 4697198..4697881 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LGM11_RS23135 (4698053) 4698053..4699438 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
LGM11_RS23140 (4699456) 4699456..4699800 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T219608 WP_023279404.1 NZ_CP084859:c4695395-4694862 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T219608 NZ_CP116557:2622090-2622233 [Enterococcus faecalis]
ATGAATGTAAGTGCTAAAATCTATGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT219608 WP_002887278.1 NZ_CP084859:c4695672-4695406 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT219608 NZ_CP116557:c2622424-2622166 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAA
TGATAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATTTTATTACTT
AAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAA
GCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References