Detailed information of TA system TA219592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 125690..126450
• Replicon: plasmid pKp_SB61170_1
• Accession: NZ_CP084857
• Organism: Klebsiella pneumoniae strain SB1170

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LGM12_RS27345
• Protein ID: WP_049157791.1
• Coordinates: 125690..126175 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LGM12_RS27350
• Protein ID: WP_110409146.1
• Coordinates: 126163..126450 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM12_RS27300 (LGM12_27300)	120875..121708	-	834	WP_087846441.1	N-6 DNA methylase	-
LGM12_RS27305 (LGM12_27305)	121754..121984	-	231	WP_086070792.1	hypothetical protein	-
LGM12_RS27310 (LGM12_27310)	122072..122419	-	348	WP_023292162.1	hypothetical protein	-
LGM12_RS27315 (LGM12_27315)	122486..122866	-	381	WP_038990973.1	hypothetical protein	-
LGM12_RS27320 (LGM12_27320)	123695..124108	-	414	WP_110409145.1	helix-turn-helix domain-containing protein	-
LGM12_RS27325 (LGM12_27325)	124109..124387	-	279	WP_047722602.1	helix-turn-helix transcriptional regulator	-
LGM12_RS27330 (LGM12_27330)	124377..124697	-	321	WP_004152720.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LGM12_RS27335 (LGM12_27335)	124852..125064	-	213	Protein_128	hypothetical protein	-
LGM12_RS27340 (LGM12_27340)	125125..125403	-	279	WP_086070787.1	hypothetical protein	-
LGM12_RS27345 (LGM12_27345)	125690..126175	-	486	WP_049157791.1	GNAT family N-acetyltransferase	Toxin
LGM12_RS27350 (LGM12_27350)	126163..126450	-	288	WP_110409146.1	DUF1778 domain-containing protein	Antitoxin
LGM12_RS27355 (LGM12_27355)	126618..126986	+	369	WP_045514389.1	DUF4440 domain-containing protein	-
LGM12_RS27360 (LGM12_27360)	127018..127521	+	504	WP_110409147.1	GrpB family protein	-
LGM12_RS27365 (LGM12_27365)	127665..127772	-	108	Protein_134	DNA mismatch repair protein MutT	-
LGM12_RS27370 (LGM12_27370)	127996..129357	+	1362	WP_049157776.1	hypothetical protein	-
LGM12_RS27375 (LGM12_27375)	129606..129736	+	131	Protein_136	integrase	-
LGM12_RS27380 (LGM12_27380)	129968..130522	+	555	WP_080895899.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167482	167482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (25-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-89)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17795.50 Da
• Isoelectric Point: 9.4841

>T219592 WP_049157791.1 NZ_CP084857:c126175-125690 [Klebsiella pneumoniae]
VGRVTAPEPLSTYHQVAEFVSGETVLDDWLKQKGLKNQALGAARTFVVCKKDTQQIAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T219592 NZ_CP116553:c2543916-2543764 [Enterococcus faecalis]
GTGGTGCGTATGAGCGCACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCT
AGGGTTTGGTATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAAAAAAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10998.73 Da
• Isoelectric Point: 4.8484

>AT219592 WP_110409146.1 NZ_CP084857:c126450-126163 [Klebsiella pneumoniae]
MLYKRCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVTGD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT219592 NZ_CP116553:2543645-2543831 [Enterococcus faecalis]
TGTGCTATAATGAAAATGAAAAGAGAGATATGAGTCAACATACCTCTCTAGTGTAGAGCCGTTTGAGACGGTGACCGATT
GTATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTTTTGTCATTTTTAAGCAATTTCACAATCAGTG
CAATCAAAGCAATGGTAAACATACCAA