Detailed information of TA system TA219591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 21017..21753
• Replicon: plasmid pKp_SB61170_1
• Accession: NZ_CP084857
• Organism: Klebsiella pneumoniae strain SB1170

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A443XD13
• Locus tag: LGM12_RS26805
• Protein ID: WP_075253142.1
• Coordinates: 21271..21753 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LGM12_RS26800
• Protein ID: WP_049083184.1
• Coordinates: 21017..21283 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM12_RS26775 (LGM12_26775)	16295..16669	-	375	WP_008786685.1	fluoride efflux transporter CrcB	-
LGM12_RS26780 (LGM12_26780)	16704..17390	-	687	WP_226614968.1	2,3-bisphosphoglycerate-dependent phosphoglycerate mutase	-
LGM12_RS26785 (LGM12_26785)	17709..18995	-	1287	WP_074091243.1	phosphopyruvate hydratase	-
LGM12_RS26790 (LGM12_26790)	19011..19439	-	429	WP_131025045.1	universal stress protein	-
LGM12_RS26795 (LGM12_26795)	19443..20405	-	963	WP_141409894.1	manganese-dependent inorganic pyrophosphatase	-
LGM12_RS26800 (LGM12_26800)	21017..21283	+	267	WP_049083184.1	DUF1778 domain-containing protein	Antitoxin
LGM12_RS26805 (LGM12_26805)	21271..21753	+	483	WP_075253142.1	GNAT family N-acetyltransferase	Toxin
LGM12_RS26810 (LGM12_26810)	21968..22234	+	267	WP_226614970.1	hypothetical protein	-
LGM12_RS26815 (LGM12_26815)	22393..23082	+	690	WP_117146692.1	YafY family protein	-
LGM12_RS26820 (LGM12_26820)	23153..23899	+	747	WP_117146690.1	SDR family NAD(P)-dependent oxidoreductase	-
LGM12_RS26825 (LGM12_26825)	24574..25176	+	603	WP_226614971.1	hypothetical protein	-
LGM12_RS26830 (LGM12_26830)	25217..26356	+	1140	WP_040107893.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167482	167482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17254.90 Da
• Isoelectric Point: 8.7400

>T219591 WP_075253142.1 NZ_CP084857:21271-21753 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T219591 NZ_CP116553:c2543493-2543389 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTTAAATT
GCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10117.61 Da
• Isoelectric Point: 4.6437

>AT219591 WP_049083184.1 NZ_CP084857:21017-21283 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDVQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT219591 NZ_CP116553:2543269-2543456 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACTTTTT
GAATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTAACAATCAGC
GCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A443XD13