Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5394598..5395223 Replicon chromosome
Accession NZ_CP084856
Organism Klebsiella pneumoniae strain SB1170

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag LGM12_RS26325 Protein ID WP_004187928.1
Coordinates 5394598..5394981 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM12_RS26330 Protein ID WP_004150355.1
Coordinates 5394981..5395223 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM12_RS26310 (5391964) 5391964..5392866 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGM12_RS26315 (5392863) 5392863..5393498 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGM12_RS26320 (5393495) 5393495..5394424 + 930 WP_004146237.1 formate dehydrogenase accessory protein FdhE -
LGM12_RS26325 (5394598) 5394598..5394981 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM12_RS26330 (5394981) 5394981..5395223 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM12_RS26335 (5395417) 5395417..5396334 + 918 WP_004187929.1 alpha/beta hydrolase -
LGM12_RS26340 (5396348) 5396348..5397289 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM12_RS26345 (5397334) 5397334..5397771 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGM12_RS26350 (5397768) 5397768..5398628 - 861 WP_004146232.1 virulence factor BrkB family protein -
LGM12_RS26355 (5398622) 5398622..5399221 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T219590 WP_004187928.1 NZ_CP084856:c5394981-5394598 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219590 NZ_CP116553:c2464972-2464871 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219590 WP_004150355.1 NZ_CP084856:c5395223-5394981 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219590 NZ_CP116553:2464748-2464934 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCG
AAAATCAGTAACGCAACAAGGGTTGCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References