Detailed information of TA system TA021959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 49213..49715
• Replicon: plasmid pSbal02
• Accession: NC_009036
• Organism: Shewanella baltica OS155

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A3DB69
• Locus tag: SBAL_RS23435
• Protein ID: WP_011839997.1
• Coordinates: 49213..49467 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A3DB70
• Locus tag: SBAL_RS23440
• Protein ID: WP_011839998.1
• Coordinates: 49464..49715 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBAL_RS23390	44486..44986	-	501	WP_011839992.1	hypothetical protein	-
SBAL_RS23395	45194..45670	+	477	WP_011839993.1	DUF417 family protein	-
SBAL_RS23400	45768..46349	-	582	WP_011839994.1	DUF2913 family protein	-
SBAL_RS23405	46346..46624	-	279	WP_011839995.1	hypothetical protein	-
SBAL_RS24255	47183..47482	+	300	WP_014620443.1	hypothetical protein	-
SBAL_RS23425	47442..48371	+	930	WP_014620444.1	integrase	-
SBAL_RS23430	48923..49198	-	276	WP_049765992.1	hypothetical protein	-
SBAL_RS23435	49213..49467	-	255	WP_011839997.1	Txe/YoeB family addiction module toxin	Toxin
SBAL_RS23440	49464..49715	-	252	WP_011839998.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SBAL_RS23445	49979..50428	+	450	WP_011839999.1	helix-turn-helix domain-containing protein	-
SBAL_RS23450	50428..50997	+	570	WP_011840000.1	PIN domain-containing protein	-
SBAL_RS23455	51060..52229	-	1170	WP_011840001.1	IS256-like element ISSba12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74000	74000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10165.67 Da
• Isoelectric Point: 9.8630

>T21959 WP_011839997.1 NC_009036:c49467-49213 [Shewanella baltica OS155]
MKILFSSNAWDDYLYWQQQDKKTLKRVNLLIKECQRAPFEGIGKPERLKENLSGYWSRRITDEHRLVYKASQDTLTIVAL
RYHY

Nucleotide

• Length: 255 bp

>T21959 NC_009036:c49467-49213 [Shewanella baltica OS155]
ATGAAAATTCTTTTTTCTTCCAACGCCTGGGATGACTACCTTTACTGGCAACAGCAGGATAAAAAAACGTTGAAACGAGT
TAATCTCCTGATTAAAGAATGCCAAAGGGCCCCATTTGAAGGTATTGGTAAACCGGAAAGATTAAAAGAAAATTTATCGG
GGTATTGGTCTCGCAGGATCACCGATGAACATCGATTAGTCTATAAGGCTAGTCAAGATACGTTGACGATCGTAGCGCTC
CGATATCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9487.57 Da
• Isoelectric Point: 4.7993

>AT21959 WP_011839998.1 NC_009036:c49715-49464 [Shewanella baltica OS155]
MAIYSFTEVRSNFKSICDTVVNDCEPVVIHRRGNENVVLFSESEFNSWKETLYLLSNPANAKRLLESIKQVNDGHASEHE
LSE

Nucleotide

• Length: 252 bp

>AT21959 NC_009036:c49715-49464 [Shewanella baltica OS155]
ATGGCTATTTACAGTTTCACAGAAGTTAGAAGTAACTTTAAGTCTATTTGCGACACTGTCGTCAATGATTGTGAGCCCGT
GGTAATACACCGTCGGGGTAACGAGAATGTAGTGTTATTTTCAGAATCTGAATTTAATAGTTGGAAGGAGACGCTATATT
TATTGTCGAACCCTGCTAATGCTAAACGTTTATTAGAATCCATAAAGCAAGTCAATGATGGACATGCTTCTGAGCATGAG
CTATCAGAATGA

Structures

Toxin

• AlphaFold DB: A3DB69

Antitoxin

• AlphaFold DB: A3DB70