Detailed information of TA system TA219589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5076102..5077783
• Replicon: chromosome
• Accession: NZ_CP084856
• Organism: Klebsiella pneumoniae strain SB1170

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: LGM12_RS24850
• Protein ID: WP_004146663.1
• Coordinates: 5076102..5077019 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: LGM12_RS24855
• Protein ID: WP_004177809.1
• Coordinates: 5077025..5077783 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM12_RS24830 (5071408)	5071408..5072928	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
LGM12_RS24835 (5072925)	5072925..5073923	+	999	WP_002885172.1	ribose ABC transporter permease	-
LGM12_RS24840 (5073954)	5073954..5074895	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LGM12_RS24845 (5074970)	5074970..5075977	+	1008	WP_009484222.1	carbohydrate kinase family protein	-
LGM12_RS24850 (5076102)	5076102..5077019	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LGM12_RS24855 (5077025)	5077025..5077783	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LGM12_RS24860 (5077869)	5077869..5078597	-	729	WP_002885152.1	response regulator transcription factor	-
LGM12_RS24865 (5078870)	5078870..5081017	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
LGM12_RS24870 (5081479)	5081479..5082165	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T219589 WP_004146663.1 NZ_CP084856:c5077019-5076102 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T219589 NZ_CP116553:c312605-312510 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGCTGGAAAA
ACAGAACGAGGAATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT219589 WP_004177809.1 NZ_CP084856:c5077783-5077025 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT219589 NZ_CP116553:312411-312475 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGCAGTAGGGTGTCTTTTTTT

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05