Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4046959..4047578 Replicon chromosome
Accession NZ_CP084856
Organism Klebsiella pneumoniae strain SB1170

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGM12_RS19785 Protein ID WP_002892050.1
Coordinates 4047360..4047578 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LGM12_RS19780 Protein ID WP_002892066.1
Coordinates 4046959..4047333 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM12_RS19770 (4042111) 4042111..4043304 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGM12_RS19775 (4043327) 4043327..4046473 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LGM12_RS19780 (4046959) 4046959..4047333 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LGM12_RS19785 (4047360) 4047360..4047578 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LGM12_RS19790 (4047737) 4047737..4048303 + 567 WP_002892030.1 maltose O-acetyltransferase -
LGM12_RS19795 (4048275) 4048275..4048415 - 141 WP_004147370.1 hypothetical protein -
LGM12_RS19800 (4048436) 4048436..4048906 + 471 WP_002892026.1 YlaC family protein -
LGM12_RS19805 (4048881) 4048881..4050331 - 1451 Protein_3884 PLP-dependent aminotransferase family protein -
LGM12_RS19810 (4050432) 4050432..4051130 + 699 WP_002892021.1 GNAT family protein -
LGM12_RS19815 (4051127) 4051127..4051267 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LGM12_RS19820 (4051267) 4051267..4051530 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219586 WP_002892050.1 NZ_CP084856:4047360-4047578 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219586 NZ_CP116549:509175-509516 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT219586 WP_002892066.1 NZ_CP084856:4046959-4047333 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219586 NZ_CP116549:508945-509175 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References