Detailed information of TA system TA021958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 40115..40671
• Replicon: plasmid pSbal02
• Accession: NC_009036
• Organism: Shewanella baltica OS155

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A3DB58
• Locus tag: SBAL_RS23360
• Protein ID: WP_011839986.1
• Coordinates: 40115..40501 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A3DB59
• Locus tag: SBAL_RS23365
• Protein ID: WP_011839987.1
• Coordinates: 40501..40671 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBAL_RS23345	35801..38785	+	2985	WP_011839984.1	Tn3-like element ISSba14 family transposase	-
SBAL_RS23350	39280..39618	-	339	WP_011839985.1	hypothetical protein	-
SBAL_RS23355	39714..40034	-	321	WP_014620440.1	hypothetical protein	-
SBAL_RS23360	40115..40501	-	387	WP_011839986.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
SBAL_RS23365	40501..40671	-	171	WP_011839987.1	hypothetical protein	Antitoxin
SBAL_RS23370	40805..41236	-	432	WP_011839988.1	DUF3577 domain-containing protein	-
SBAL_RS23375	41541..43664	-	2124	WP_011839989.1	DNA topoisomerase III	-
SBAL_RS23380	43674..43985	-	312	WP_011839990.1	hypothetical protein	-
SBAL_RS23385	43988..44437	-	450	WP_011839991.1	single-stranded DNA-binding protein	-
SBAL_RS23390	44486..44986	-	501	WP_011839992.1	hypothetical protein	-
SBAL_RS23395	45194..45670	+	477	WP_011839993.1	DUF417 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74000	74000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (10-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14302.17 Da
• Isoelectric Point: 3.9061

>T21958 WP_011839986.1 NC_009036:c40501-40115 [Shewanella baltica OS155]
MNFIQFPAERVVEINQAILLNEPGFRGEYDIGRLSGALSRIDNAIAYEGLDDVFLIAAKYATSVAMAHAFSDANKRTGLA
LCLEYLSLNDYEINTEYDELSDAMVDLVVGTLSEIEFADVLYSMWRSM

Nucleotide

• Length: 387 bp

>T21958 NC_009036:c40501-40115 [Shewanella baltica OS155]
ATGAATTTCATTCAGTTTCCTGCTGAACGAGTCGTTGAAATCAATCAGGCCATTCTGTTGAATGAACCAGGTTTTCGCGG
TGAATATGATATCGGCAGATTGTCGGGTGCGTTATCGCGGATCGACAATGCTATTGCTTATGAAGGCTTGGATGATGTGT
TTTTAATTGCGGCTAAATATGCGACTTCAGTCGCTATGGCACACGCTTTTTCAGATGCAAACAAGCGCACAGGGCTTGCC
TTGTGTTTAGAGTATTTGTCACTCAATGATTACGAAATAAACACTGAGTATGATGAATTATCGGATGCAATGGTTGATTT
AGTTGTTGGTACACTAAGCGAAATCGAATTTGCAGATGTTCTGTATTCAATGTGGCGATCAATGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6390.54 Da
• Isoelectric Point: 10.7315

>AT21958 WP_011839987.1 NC_009036:c40671-40501 [Shewanella baltica OS155]
MLNETKKYGVNIVERPHVKANKKLDLTGGCGKQIVHSETKLVLRTHKNTFRKLADM

Nucleotide

• Length: 171 bp

>AT21958 NC_009036:c40671-40501 [Shewanella baltica OS155]
ATGTTAAATGAAACAAAGAAATACGGTGTAAACATCGTAGAGCGTCCACATGTGAAAGCCAATAAAAAGCTTGATTTGAC
TGGTGGTTGCGGTAAGCAGATTGTGCATAGCGAAACAAAACTTGTTTTACGAACGCATAAAAATACTTTTCGCAAACTGG
CTGATATGTAA

Structures

Toxin

• AlphaFold DB: A3DB58

Antitoxin

• AlphaFold DB: A3DB59