Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73955..74598 Replicon plasmid pKp_SB62390_1
Accession NZ_CP084853
Organism Klebsiella pneumoniae strain SB2390

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LGM13_RS26295 Protein ID WP_000754566.1
Coordinates 73955..74371 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LGM13_RS26300 Protein ID WP_001261276.1
Coordinates 74368..74598 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM13_RS26270 (LGM13_26270) 69791..70618 - 828 WP_004152694.1 phosphonate ABC transporter, permease protein PhnE -
LGM13_RS26275 (LGM13_26275) 70627..71490 - 864 WP_004152695.1 phosphate/phosphite/phosphonate ABC transporter substrate-binding protein -
LGM13_RS26280 (LGM13_26280) 71487..72314 - 828 WP_004153729.1 phosphonate ABC transporter ATP-binding protein -
LGM13_RS26285 (LGM13_26285) 72656..72964 + 309 Protein_78 IS5/IS1182 family transposase -
LGM13_RS26290 (LGM13_26290) 73170..73874 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LGM13_RS26295 (LGM13_26295) 73955..74371 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM13_RS26300 (LGM13_26300) 74368..74598 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LGM13_RS26305 (LGM13_26305) 75187..75480 + 294 WP_020956883.1 hypothetical protein -
LGM13_RS26310 (LGM13_26310) 75544..76230 + 687 WP_020956882.1 hypothetical protein -
LGM13_RS26315 (LGM13_26315) 76242..77018 + 777 WP_020956881.1 tyrosine-type recombinase/integrase -
LGM13_RS26320 (LGM13_26320) 77076..77333 - 258 WP_000764642.1 hypothetical protein -
LGM13_RS26325 (LGM13_26325) 77462..77566 - 105 WP_032409716.1 hypothetical protein -
LGM13_RS26330 (LGM13_26330) 78096..78962 + 867 WP_004118283.1 replication initiation protein -
LGM13_RS26335 (LGM13_26335) 79139..79408 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / dfrA12 / aadA2 / qacE / sul1 / mph(A) / blaSHV-12 / aac(6')-Il / ant(3'')-Ia / blaTEM-1A - 1..196349 196349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T219574 WP_000754566.1 NZ_CP084853:c74371-73955 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T219574 NZ_CP116536:517080-517421 [Enterococcus faecium]
GTGTCAGGAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT219574 WP_001261276.1 NZ_CP084853:c74598-74368 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT219574 NZ_CP116536:516850-517080 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGACGATGGTACAATTACGTTAGTTCCAAAAATTAAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References