Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5268628..5269253 Replicon chromosome
Accession NZ_CP084852
Organism Klebsiella pneumoniae strain SB2390

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGM13_RS25535 Protein ID WP_002882817.1
Coordinates 5268628..5269011 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGM13_RS25540 Protein ID WP_004150355.1
Coordinates 5269011..5269253 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM13_RS25520 (5265994) 5265994..5266896 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGM13_RS25525 (5266893) 5266893..5267528 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LGM13_RS25530 (5267525) 5267525..5268454 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGM13_RS25535 (5268628) 5268628..5269011 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM13_RS25540 (5269011) 5269011..5269253 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGM13_RS25545 (5269458) 5269458..5270375 + 918 WP_004181612.1 alpha/beta hydrolase -
LGM13_RS25550 (5270389) 5270389..5271330 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LGM13_RS25555 (5271375) 5271375..5271812 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LGM13_RS25560 (5271809) 5271809..5272669 - 861 WP_004146232.1 virulence factor BrkB family protein -
LGM13_RS25565 (5272663) 5272663..5273262 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219572 WP_002882817.1 NZ_CP084852:c5269011-5268628 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219572 NZ_CP116532:479747-480088 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219572 WP_004150355.1 NZ_CP084852:c5269253-5269011 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219572 NZ_CP116532:479517-479747 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References