Detailed information of TA system TA219566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2783057..2784645
• Replicon: chromosome
• Accession: NZ_CP084852
• Organism: Klebsiella pneumoniae strain SB2390

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LGM13_RS13820
• Protein ID: WP_004179815.1
• Coordinates: 2783057..2784379 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LGM13_RS13825
• Protein ID: WP_004176198.1
• Coordinates: 2784379..2784645 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM13_RS13805 (2778113)	2778113..2780614	+	2502	WP_004179823.1	fimbria/pilus outer membrane usher protein	-
LGM13_RS13810 (2780602)	2780602..2781606	+	1005	WP_032443455.1	fimbrial protein	-
LGM13_RS13815 (2781748)	2781748..2783040	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LGM13_RS13820 (2783057)	2783057..2784379	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
LGM13_RS13825 (2784379)	2784379..2784645	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LGM13_RS13830 (2784911)	2784911..2785237	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LGM13_RS13835 (2785234)	2785234..2785734	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LGM13_RS13840 (2785712)	2785712..2787091	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
LGM13_RS13845 (2787437)	2787437..2788591	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LGM13_RS13850 (2788572)	2788572..2789495	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2583918..2819480	235562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T219566 WP_004179815.1 NZ_CP084852:c2784379-2783057 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T219566 NZ_CP116523:189064-189330 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT219566 WP_004176198.1 NZ_CP084852:c2784645-2784379 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT219566 NZ_CP116523:188801-189064 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA