Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20733..21376 Replicon plasmid pKp_SB611_4
Accession NZ_CP084847
Organism Klebsiella pneumoniae strain SB611

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag LGM15_RS28515 Protein ID WP_020804312.1
Coordinates 20733..21149 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LGM15_RS28520 Protein ID WP_001261276.1
Coordinates 21146..21376 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM15_RS28495 (LGM15_28495) 15930..16955 - 1026 WP_064782720.1 IS110 family transposase -
LGM15_RS28500 (LGM15_28500) 17031..17885 - 855 WP_226651245.1 cysteine peptidase family C39 domain-containing protein -
LGM15_RS28505 (LGM15_28505) 17875..19152 - 1278 WP_009309915.1 HlyD family secretion protein -
LGM15_RS28510 (LGM15_28510) 19814..20692 + 879 WP_009309916.1 restriction endonuclease -
LGM15_RS28515 (LGM15_28515) 20733..21149 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
LGM15_RS28520 (LGM15_28520) 21146..21376 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LGM15_RS28525 (LGM15_28525) 21964..22908 + 945 WP_011977810.1 hypothetical protein -
LGM15_RS28530 (LGM15_28530) 22945..23337 + 393 WP_011977811.1 hypothetical protein -
LGM15_RS28535 (LGM15_28535) 23395..23916 + 522 WP_011977812.1 hypothetical protein -
LGM15_RS28540 (LGM15_28540) 23962..24165 + 204 WP_011977813.1 hypothetical protein -
LGM15_RS28545 (LGM15_28545) 24195..25199 + 1005 WP_011977814.1 hypothetical protein -
LGM15_RS28550 (LGM15_28550) 25383..26162 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49648 49648


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T219555 WP_020804312.1 NZ_CP084847:c21149-20733 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T219555 NZ_CP116496:c572506-572243 [Rickettsia asembonensis]
ATGATTGAGATTGAGTCGACACTAGAAGCAGCGGAGGACTTAGCGTATTGGAAAAAATACGATATTAAAAAATATGAACG
TATTAAACTTCTAATAAAAAATATCCAAGAAGCACCGTTTACAGGTATAGGTAAGCCCGAACCTTTAAAACATATATTAT
CAGGTTTATGGTCACGTAGAATTAACCACGAACATAGACTAATATATTCTGTCAATCCTAAACAAATTATAATATATAAT
TGTAGATTTCATTATAAAAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT219555 WP_001261276.1 NZ_CP084847:c21376-21146 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT219555 NZ_CP116496:c572750-572499 [Rickettsia asembonensis]
ATGAACTCAATTAGCGGCACTTCATTTAGAAAAAACTTAAGCTCTGTACTAAATACCGTAGAAAACGATCATGTCCCTTA
TCTTATTAAAAGAAAGAATCATAAGAATATTATTCTTTTAACCGAAGAAGAATATGAATCTACAAAAGAAACATTATATT
TATTATCTAATCCGGCTAATGCTAATCGAATAAAAAAATCTATCGAACAAGCAAAAAGAGGAGAATTTGCTAAAGTTAAT
TTAGATGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References