Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 39360..39885 Replicon plasmid pKp_SB611_3
Accession NZ_CP084846
Organism Klebsiella pneumoniae strain SB611

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag LGM15_RS28055 Protein ID WP_004197633.1
Coordinates 39580..39885 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LGM15_RS28050 Protein ID WP_001568025.1
Coordinates 39360..39578 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM15_RS28035 (LGM15_28035) 36119..36511 - 393 Protein_34 IS630 family transposase -
LGM15_RS28040 (LGM15_28040) 36581..37561 + 981 WP_000019403.1 IS5-like element IS5 family transposase -
LGM15_RS28045 (LGM15_28045) 37792..38766 + 975 WP_110243103.1 sensor domain-containing diguanylate cyclase -
LGM15_RS28050 (LGM15_28050) 39360..39578 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LGM15_RS28055 (LGM15_28055) 39580..39885 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
LGM15_RS28060 (LGM15_28060) 40054..40449 + 396 WP_017899885.1 hypothetical protein -
LGM15_RS28065 (LGM15_28065) 40476..40790 + 315 WP_053389906.1 hypothetical protein -
LGM15_RS28070 (LGM15_28070) 40801..41817 + 1017 WP_017899884.1 hypothetical protein -
LGM15_RS28075 (LGM15_28075) 42015..42809 + 795 WP_042929975.1 site-specific integrase -
LGM15_RS28080 (LGM15_28080) 43305..43607 - 303 WP_071571079.1 hypothetical protein -
LGM15_RS28085 (LGM15_28085) 43604..44230 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..101892 101892
- flank IS/Tn - - 36581..37561 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T219554 WP_004197633.1 NZ_CP084846:39580-39885 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T219554 NZ_CP116496:334877-335158 [Rickettsia asembonensis]
ATGAGAACGATTAAACGTACTGCACAGTTTAAACGTGATTATAAGCGTGAAAAACGTAGAAAACACGTAATAAATTTGGA
CGATATTTTATTAAAAGCAGTTAAGTACTTGATTGCTGATACAACATTACCTGTAAATATGAGAGATCATGCTCTTATAG
GTAATTGGAAAGATTGTAGAGATTGTCATATTAAACCAGATTTAGTACTAATTTATAGAAAACCCGATGCTGACACATTG
GAATTAATACGATTAGGCTCACACAATGAACTTGGTTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT219554 WP_001568025.1 NZ_CP084846:39360-39578 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT219554 NZ_CP116496:334627-334890 [Rickettsia asembonensis]
ATGTCTGCTGATTCTATTGTGCGAGCCCGTATTAACGAAGATGTAAAAGAAGAAGCAGCCTTAGTACTTGCTGCTATGGG
GCTTACTTTATCTGATGCTGTTCGTATGCTTTTATTTCGTGTTGCTCGTGAAAAAGCATTGCCGTTTGAACCGCTTATCC
CAAATGATGAAACAATTAAGGCAATGAAAGCTGCAAGAAGCGGTAAATTAGTTCATGTTGGTAATATAAATAATTTTTTG
TCCGATTTAAATGAGAACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References