Detailed information of TA system TA219552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 24207..24943
• Replicon: plasmid pKp_SB611_1
• Accession: NZ_CP084844
• Organism: Klebsiella pneumoniae strain SB611

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LGM15_RS26525
• Protein ID: WP_003026803.1
• Coordinates: 24207..24689 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LGM15_RS26530
• Protein ID: WP_003026799.1
• Coordinates: 24677..24943 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM15_RS26480 (LGM15_26480)	19291..19686	-	396	Protein_21	IS30 family transposase	-
LGM15_RS26485 (LGM15_26485)	19751..20455	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LGM15_RS26490 (LGM15_26490)	20510..20704	-	195	Protein_23	helix-turn-helix domain-containing protein	-
LGM15_RS26495 (LGM15_26495)	20908..21171	-	264	WP_049015287.1	hypothetical protein	-
LGM15_RS26500 (LGM15_26500)	21186..21449	+	264	WP_162556057.1	transposase	-
LGM15_RS26505 (LGM15_26505)	21394..21558	+	165	Protein_26	transposase domain-containing protein	-
LGM15_RS26510 (LGM15_26510)	21946..22251	-	306	WP_230354784.1	hypothetical protein	-
LGM15_RS26515 (LGM15_26515)	22405..23109	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
LGM15_RS26520 (LGM15_26520)	23143..24006	-	864	WP_267462104.1	ISNCY family transposase	-
LGM15_RS26525 (LGM15_26525)	24207..24689	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LGM15_RS26530 (LGM15_26530)	24677..24943	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LGM15_RS26535 (LGM15_26535)	25388..25618	-	231	WP_004206660.1	hypothetical protein	-
LGM15_RS26540 (LGM15_26540)	25632..25835	-	204	WP_004206661.1	HHA domain-containing protein	-
LGM15_RS26545 (LGM15_26545)	25896..26387	-	492	WP_004206662.1	hypothetical protein	-
LGM15_RS26550 (LGM15_26550)	26521..27732	-	1212	WP_021312522.1	hypothetical protein	-
LGM15_RS26555 (LGM15_26555)	27729..27977	-	249	WP_004193994.1	hypothetical protein	-
LGM15_RS26560 (LGM15_26560)	28335..28685	+	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
LGM15_RS26565 (LGM15_26565)	28737..29099	+	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / mrkA	1..155131	155131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T219552 WP_003026803.1 NZ_CP084844:c24689-24207 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T219552 NZ_CP116494:76902-77051 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT219552 WP_003026799.1 NZ_CP084844:c24943-24677 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT219552 NZ_CP116494:c76857-76798 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66