Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4028125..4028744 Replicon chromosome
Accession NZ_CP084843
Organism Klebsiella pneumoniae strain SB611

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LGM15_RS20075 Protein ID WP_002892050.1
Coordinates 4028526..4028744 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LGM15_RS20070 Protein ID WP_002892066.1
Coordinates 4028125..4028499 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM15_RS20060 (4023278) 4023278..4024471 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGM15_RS20065 (4024494) 4024494..4027640 + 3147 WP_226650890.1 multidrug efflux RND transporter permease subunit AcrB -
LGM15_RS20070 (4028125) 4028125..4028499 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LGM15_RS20075 (4028526) 4028526..4028744 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LGM15_RS20080 (4028907) 4028907..4029473 + 567 WP_002892030.1 maltose O-acetyltransferase -
LGM15_RS20085 (4029445) 4029445..4029585 - 141 WP_004147370.1 hypothetical protein -
LGM15_RS20090 (4029606) 4029606..4030076 + 471 WP_002892026.1 YlaC family protein -
LGM15_RS20095 (4030051) 4030051..4031502 - 1452 WP_159211870.1 PLP-dependent aminotransferase family protein -
LGM15_RS20100 (4031603) 4031603..4032301 + 699 WP_002892021.1 GNAT family protein -
LGM15_RS20105 (4032298) 4032298..4032438 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LGM15_RS20110 (4032438) 4032438..4032701 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219547 WP_002892050.1 NZ_CP084843:4028526-4028744 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219547 NZ_CP116492:120122-120565 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT219547 WP_002892066.1 NZ_CP084843:4028125-4028499 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219547 NZ_CP116492:119895-120125 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References