Detailed information of TA system TA219546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2743326..2744914
• Replicon: chromosome
• Accession: NZ_CP084843
• Organism: Klebsiella pneumoniae strain SB611

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LGM15_RS13780
• Protein ID: WP_096812286.1
• Coordinates: 2743326..2744648 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LGM15_RS13785
• Protein ID: WP_002904790.1
• Coordinates: 2744648..2744914 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM15_RS13765 (2738382)	2738382..2740883	+	2502	WP_226650804.1	fimbria/pilus outer membrane usher protein	-
LGM15_RS13770 (2740871)	2740871..2741875	+	1005	WP_032421320.1	fimbrial protein	-
LGM15_RS13775 (2742017)	2742017..2743309	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LGM15_RS13780 (2743326)	2743326..2744648	-	1323	WP_096812286.1	type II toxin-antitoxin system HipA family toxin	Toxin
LGM15_RS13785 (2744648)	2744648..2744914	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LGM15_RS13790 (2745180)	2745180..2745506	-	327	WP_226650805.1	hydroxyisourate hydrolase	-
LGM15_RS13795 (2745503)	2745503..2746003	-	501	WP_048999765.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LGM15_RS13800 (2745981)	2745981..2747360	-	1380	WP_002904785.1	nucleobase:cation symporter-2 family protein	-
LGM15_RS13805 (2747706)	2747706..2748860	+	1155	WP_159211758.1	FAD-dependent urate hydroxylase HpxO	-
LGM15_RS13810 (2748841)	2748841..2749764	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2544368..2789720	245352

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49095.41 Da
• Isoelectric Point: 7.5366

>T219546 WP_096812286.1 NZ_CP084843:c2744648-2743326 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T219546 NZ_CP116491:c5444089-5443706 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT219546 WP_002904790.1 NZ_CP084843:c2744914-2744648 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT219546 NZ_CP116491:c5444331-5444089 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8