Detailed information of TA system TA219534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 343459..344045
• Replicon: chromosome
• Accession: NZ_CP084843
• Organism: Klebsiella pneumoniae strain SB611

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LGM15_RS01640
• Protein ID: WP_002920800.1
• Coordinates: 343677..344045 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LGM15_RS01635
• Protein ID: WP_004174006.1
• Coordinates: 343459..343680 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM15_RS01615 (339616)	339616..340542	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LGM15_RS01620 (340539)	340539..341816	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LGM15_RS01625 (341813)	341813..342580	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LGM15_RS01630 (342582)	342582..343295	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LGM15_RS01635 (343459)	343459..343680	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LGM15_RS01640 (343677)	343677..344045	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LGM15_RS01645 (344318)	344318..345634	+	1317	WP_080899742.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LGM15_RS01650 (345741)	345741..346628	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LGM15_RS01655 (346625)	346625..347470	+	846	WP_004174009.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LGM15_RS01660 (347472)	347472..348542	+	1071	WP_159211797.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	338445..351492	13047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T219534 WP_002920800.1 NZ_CP084843:343677-344045 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T219534 NZ_CP116490:1332282-1332530 [Haemophilus influenzae]
ATGGGACGAACTGAAAAATTATTAGATAAGCTCGCACAATCAAAATCTACATTTAATTGGAATGAATTAGTTTCTTTGTT
AGCTCAACAAGGTTATGAAAAGCGAGAAATGGCAGGCTCTCGAGTGAGATTTTATAATAGAACACTCGAACATACGATTT
TGTTACACAAGCCTCACCCTGAAAATTATATTAAAGGTGGTGCTTTAAAGTCAGTGAAAGAATCATTAAAACAGGTAGGT
ATTCTATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT219534 WP_004174006.1 NZ_CP084843:343459-343680 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT219534 NZ_CP116490:1332527-1332871 [Haemophilus influenzae]
ATGAAGTTATTAAATTATAAAGGTTATGTTGGCACGATTGAGGCGGATTTAGAAAACAATATATTGTTTGGCAAACTTGC
TTACATTCGTGATTTAGTGACTTACGAAGCAGAGTCATTATCTGAGCTAGAAAAAGAATTTCGTCAATCTGTTGATTTAT
ATTTACAAGATTGTTTGGAATTAGGTAAAGAACCGAATAAGCCTTTTAAAGGTGTATTTAATGTACGAATTGGCGAGGAA
TTGCATAGAGAAGCTACGATCATAGCAGGCGATCGTTCTCTTAATGCTTTTGTTACGGAAGCGATTCAAGAAAAAATTTT
TCGTGAAAAACCAAGTTTAAGATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7